Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Transgenic Organisms00:53

Transgenic Organisms

Overview
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Transgenic Organisms00:53

Transgenic Organisms

Overview
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

SnakeAltPromoter Facilitates Differential Alternative Promoter Analysis.

Computational and structural biotechnology journal·2026
Same author

Publisher Correction: Herpesviruses mimic zygotic genome activation to promote viral replication.

Nature communications·2025
Same author

Herpesviruses mimic zygotic genome activation to promote viral replication.

Nature communications·2025
Same author

MSL2 ensures biallelic gene expression in mammals.

Nature·2023
Same journal

Genetic survey of biomarkers at early and mid-pregnancy identifies pregnancy-specialized immune regulation.

PLoS genetics·2026
Same journal

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program.

PLoS genetics·2026
Same journal

Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

PLoS genetics·2026
Same journal

Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

PLoS genetics·2026
Same journal

Multiple instance fine-mapping: Predicting causal regulatory variants with a deep sequence model.

PLoS genetics·2026
Same journal

Nuclear ubiquitin-conjugating enzyme TrUbc4 and F-box protein TrFwd1-mediated modification of Cre1 in Trichoderma reesei establishes a regulatory mechanism for carbon catabolite repression.

PLoS genetics·2026
查看所有相关文章

相关实验视频

Updated: Jun 17, 2026

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
08:59

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Published on: November 2, 2015

在器官发育过程中使用替代促进剂.

Jiang Tan1,2,3, Yidan Sun1,2,3

  • 1Department of Genetics, Washington University School of Medicine, St. Louis, Missouri, United States of America.

PLoS genetics
|March 28, 2025
PubMed
概括
此摘要是机器生成的。

替代性促进剂通过启用独特的基因表达模式来推动器官的发育和功能. 这项研究揭示了它们在哺乳动物发育过程中塑造器官身份的关键,经常被忽视的作用.

更多相关视频

Technique to Target Microinjection to the Developing Xenopus Kidney
11:29

Technique to Target Microinjection to the Developing Xenopus Kidney

Published on: May 3, 2016

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

相关实验视频

Last Updated: Jun 17, 2026

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
08:59

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

Published on: November 2, 2015

Technique to Target Microinjection to the Developing Xenopus Kidney
11:29

Technique to Target Microinjection to the Developing Xenopus Kidney

Published on: May 3, 2016

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

科学领域:

  • 发展生物学 发展生物学
  • 基因组学就是基因组学.
  • 分子生物学分子生物学

背景情况:

  • 动态基因表达对于哺乳动物器官的发育和功能至关重要.
  • 许多基因利用多个促进器,但替代性促进器在有机生成中的作用尚不清楚.

研究的目的:

  • 研究替代促进体在哺乳动物器官发育中的作用.
  • 识别和描述各种器官和发育阶段的发育动态促进体 (DDP).

主要方法:

  • 分析了来自不同发育阶段和七个主要器官的313只小鼠样本的RNA测序 (RNA-seq) 数据.
  • 确定活跃的发起人,区分主要和替代发起人.
  • 对替代促进剂使用的监管和功能影响的分析.

主要成果:

  • 在每种器官中,分别发现了967到3237个发育动态促进物 (DDP).
  • 发现主要和替代性促进者都具有发展动态.
  • 替代性促进体可以独立调节,对特定器官的功能和基因表达至关重要.
  • 增加替代性促进剂的使用显著影响器官的身份和功能.

结论:

  • 替代性促进剂的使用是哺乳动物器官发育中的关键,但经常被忽视的机制.
  • 这些发现为器官生成的监管复杂性和促进体多样性的功能意义提供了新的见解.