猪肉加工设施中的沙门氏菌的临时性和遗传相关性
A E Asmus1, K M Heimer2, K W Davis2
1Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN 55108, USA; Hormel Foods Corporation, Austin, MN 55912, USA.
Journal of food protection
|March 28, 2025
概括
猪肉上的沙门氏菌发病率高于表面的沙门氏菌,特定的血清型如沙门氏菌I4,[5],12:i:-在加工线和日期中持续存在. 这些发现表明在加工前有一个常见的污染源.
科学领域:
- 食品安全微生物学 食品安全微生物学
- 兽医公共卫生 兽医公共卫生
- 基因组流行病学基因组流行病学
背景情况:
- 沙门氏菌 (Salmonella enterica) 是一种重要的食物传播病原体.
- 猪肉加工厂是减少沙门氏菌污染的关键控制点.
- 了解沙门氏菌的流行和遗传多样性对于有效的控制策略至关重要.
研究的目的:
- 为了确定猪肉加工环境中Salmonella enterica的流行率和遗传相关性.
- 在两条加工线上调查肉类和接触表面的沙门氏菌污染情况.
- 分析商业生产期间沙门氏菌血清型的时间和空间分布.
主要方法:
- 从两个猪肉加工线 (Bootjack Trim和Boston Butt Trim) 取肉和接触面的样本.
- 在192个样本中量化沙门氏菌的流行率.
- 使用核心单核酸多形态 (SNP) 的基因分析来评估分离物的遗传相关性.
- 已识别的沙门氏菌株与公开可用的基因组进行比较.
主要成果:
- 牛肉和BBT肉 (11.5%) 之间沙门氏菌发病率没有显著差异.
- 与接触面相比,肉类中的沙门氏菌发病率更高,这两条加工线都是如此.
- 在四个不同的加工窗口内聚类沙门氏菌的流行率和血清型.
- 在加工线和日期中识别出高度相关的沙门氏菌I4,[5],12:i:-,沙门氏菌伦敦,沙门氏菌Typhimurium和沙门氏菌阿戈纳菌株.
- 与其他植物的分离物相比,具有遗传特异性的沙门氏菌I4,[5],12:i:-和伦敦菌株.
结论:
- 沙门氏菌的流行程度因加工线和生产计划而异.
- 检测到的沙门氏菌血清型的高度遗传相关性表明在加工前有一个共同来源.
- 需要持续监测和有针对性的干预措施来控制猪肉加工设施中的沙门氏菌.
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