斯特里格马托西斯对猪卵细胞成熟和随后胚胎发育的毒性影响
Yumin Lee1, Dabin Cha1, Seunghyun Choi1
1Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
Toxicology letters
|March 28, 2025
概括
雌激素 (STE) 对猪卵细胞成熟和胚胎发育产生负面影响. 这种真菌毒素诱导氧化应激,线粒体功能障碍,DNA损伤和亡,突出显示其生殖毒性.
科学领域:
- 生殖生物学 生殖生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 斯特里格马托西斯 (STE) 是一种真菌毒素和亚拉托辛B1前体.
- 之前的研究重点是亚黄素B1的毒性,对STE对卵细胞成熟的影响的数据有限.
研究的目的:
- 调查菌囊素 (STE) 对猪卵细胞成熟和随后胚胎发育的影响.
- 阐明STE诱导的卵细胞毒性的潜在细胞机制.
主要方法:
- 猪卵细胞在实验室中经历了不同度的STE成熟 (0,5,10,25μM).
- 评估了累积膨胀,核成熟 (二次元期的速度) 和胚胎发育 (胚胎细胞形成).
- 评估了氧化应激标志物 (ROS,谷氨),线粒体功能,DNA损伤 (γ-H2AX),自 (LC3) 和亡 (Annexin-V).
主要成果:
- STE显著降低了累积扩张和二期转化率,具有剂量依赖的效应.
- 在STE度超过10μM时,胚胎细胞形成率显著下降.
- 暴露于STE增加了活性氧物种,DNA损伤,自和亡,同时减少了谷氨,线粒体数量和膜潜力.
结论:
- 斯特里格马托西斯会影响猪卵细胞的成熟和胚胎的发育.
- 通过氧化应激,线粒体功能障碍,DNA损伤,过度自和早期亡,STE会产生毒性.
- 这些发现凸显了与动物料中STE污染相关的生殖风险.
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