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Updated: May 8, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

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不同的方法将HLA类I分子从细菌包容体中分离出来,形成异构复合体

Dalton Kiefer1, Lucas Bierscheid2, Oliver Kask3

  • 1Center for Applied Structural Discovery, Biodesign Institute at Arizona State University, Tempe, AZ, 85281, USA; Center for Personalized Diagnostics, Biodesign Institute at Arizona State University, Tempe, AZ, 85281, USA; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85281, USA.

Protein expression and purification
|March 28, 2025
PubMed
概括

改善复合人类白细胞抗原I类 (HLA-I) 蛋白质的重新折叠是免疫学的关键. 在添加重链和之前,先氧化β2m轻链,可显著提高HLA-I复合体组装效率.

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相关概念视频

Antigen Processing Pathways01:31

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...