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对表型方法进行比较,以证明Staphylococcus aureus中的青素敏感性

Ravin Hettiarachchi¹, Julie Allerton², Christopher McIver³

¹Department of Microbiology, New South Wales Health Pathology, St George Hospital, Sydney, NSW, Australia; School of Biomedical Sciences, University of NSW, Sydney, NSW, Australia.

|March 28, 2025

概括

型方法在黄金杆菌的青素敏感性显示磁盘扩散测试是可靠的,不像MIC方法有高的错误率. 使用CLSI,EUCAST或CDS进行磁盘扩散测试可以确保临床实验室的准确结果.

科学领域:

临床微生物学临床微生物学
抗微生物耐药性抗微生物耐药性
分子诊断学分子诊断学

背景情况:

精确确定黄金葡萄球菌 (Staphylococcus aureus) 青素敏感性对于有效治疗至关重要.
现型方法是常用的,但与分子方法相比,它们的可靠性需要评估.

研究的目的:

评估表型方法的性能,用于对金黄色葡萄球菌 (Staphylococcus aureus) 的青素敏感性测试.
为了将光盘扩散测试和最小抑制度 (MIC) 方法与聚合酶链反应 (PCR) 进行比较,作为参考.

主要方法:

126个金黄色葡萄球菌分离物使用微稀释 (BMD) 和稀释 (AD) 进行了测试,以确定MIC.
磁盘扩散测试使用校准二分感应 (CDS),临床实验室标准研究所 (CLSI) 和欧洲抗菌感应性测试委员会 (EUCAST) 的指导方针进行.
分子测试 (blaZ和mecA PCR) 用于将其分类为易受青素感染的金黄色菌 (PSSA),易受甲素感染的金黄色菌 (MSSA) 和耐甲素感染的金黄色菌 (MRSA).

主要成果:

微稀释和稀释方法显示了很高的非常重大误差 (VME) 率 (9.6%),其中20%的耐药分离物被错误分类.
磁盘传播方法显示没有VMEs和高分类同意率:CLSI (98.6%),CDS (97.6%) 和EUCAST (93.8%).
独立读者的共识报告改善了所有光盘扩散方法的CA.

结论:

使用CLSI,EUCAST或CDS方法的磁盘扩散测试是报告临床黄金葡萄球菌分离物中的青素敏感性的可靠方法.
单独确定最小抑制度 (MIC) 不建议在没有确认的情况下证明青素敏感性.
通过多个读者的共识报告可以提高磁盘扩散测试的准确性.

关键词:

黄金葡萄球菌黄金葡萄球菌对抗生素敏感性测试试验.青素是一种青素.聚合酶连锁反应的发生.

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