相关实验视频
Updated: May 8, 2026

10:42
Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
微生物组的景观在不同的阶段的根腐烂的腐烂
Mengzhen Ji1, Kaixin Xiong1, Di Fu1
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
Clinical oral investigations
|March 29, 2025
概括
根 кариес涉及口腔微生物的不平衡,而不仅仅是多样性变化. 菌,Streptococcus mutans和Actinomyces viscosus之间的相互作用通过阿尔金因生物合成促进空腔的形成.
科学领域:
- 口腔微生物学 口腔微生物学
- 牙虫研究 牙虫研究
- 微生物生态学 微生物生态学
背景情况:
- 根是一种复杂的口腔疾病.
- 了解根的微生物动态对于有效治疗至关重要.
- 以前的研究还没有完全阐明根的不同阶段的微生物景观.
研究的目的:
- 为了研究微生物和根之间的关系.
- 为了确定核心微生物物种,涉及到根的发展.
- 探索关键微生物之间的相互作用及其对菌性的影响.
主要方法:
- 从表面和深层根以及健全的根表面收集了斑块样本.
- 使用16SrRNA和18SrRNA高通量测序进行微生物分析.
- 建立了体外生物膜模型来研究微生物相互作用和毒性因素.
主要成果:
- 鉴定了特定的细菌属 (Streptococcus,Actinomyces,Lactobacillus,Bacillus,Prevotella) 在不同病阶段进行丰富.
- 香白菌占主导地位,与突变性菌株和Actinomyces相关.
- 虫 Candida albicans 通过氨酸生物合成促进了S. mutans和A. viscosus的生物膜形成和化性.
结论:
- 口腔微生态相对稳定,物种不平衡导致根.
- 跨王国的相互作用,特别是涉及C. albicans,S. mutans和A. viscosus,增强了空性生物膜.
- 针对这些微生物相互作用,为预防和治疗根提供了一个有希望的策略.
相关概念视频
The Skin Microbiota
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
The Oral Microbiota
The oral microbiome includes a complex ecosystem comprising over 700 microbial species, identified through genomic sequencing and culture-based analyses to date. This community includes a core microbiome, found universally among individuals, and a variable component influenced by environmental factors such as diet, lifestyle, and host genetics. Site-specific conditions, including oxygen gradients, pH levels, and nutrient availability, determine the spatial distribution of these microorganisms...
Development of the Oral Microbiota
The establishment of the oral microbiome begins before birth, challenging the long-held belief that the fetal oral cavity is sterile. The presence of oral microbes such as Streptococcus and Fusobacterium in amniotic fluid suggests that microbial exposure may occur in utero, potentially through translocation from the maternal oral or gastrointestinal tract. This early colonization primes the neonatal immune system and sets the stage for subsequent microbial succession. Maternal health,...
Microbiota of the Respiratory Tract
The human respiratory tract, comprising the upper and lower segments, serves as a critical interface with the external environment. The upper respiratory tract (URT)—including the nostrils, sinuses, pharynx, and oropharynx—is heavily colonized by microbes, while the lower respiratory tract (LRT), composed of the larynx, trachea, bronchi, and lungs, was long thought to be sterile. However, recent molecular studies have revealed that the lungs are not devoid of microbes but act more like...
Microbiota of the Large Intestine
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
Microbiota of the Urogenital Tract
The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...

