对野生和栽培的Agaricus bisporus的风味变异的转录和代谢学见解
Zhi-Xin Cai1, Zhi-Heng Zeng1, Wen-Zhi Chen1
1Institute of Edible Mushroom, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian, China.
Scientific reports
|March 29, 2025
概括
这项研究揭示了种植Agaricus bisporus如何改变它的味道和营养. 野生和栽培类型之间的关键基因和代谢物不同,为培育更好的提供了目标.
科学领域:
- 的基因组学和代谢学.
- 可食用的种植方式
- 食品科学与营养 食品科学与营养
背景情况:
- Agaricus bisporus是一种全球种植的食用真菌.
- 野生和栽培品种之间的风味和营养特征的差异并未得到充分理解.
- 这种知识差距阻碍了培育高质量的品种.
研究的目的:
- 阐明野生类型和栽培类型Agaricus bisporus之间的风味分歧的分子和代谢基础.
- 为了确定关键的基因和代谢物,负责特征差异.
- 为了提供分子点用于培育改进的菌株.
主要方法:
- 对43种A. bisporus菌株 (23种野生型,20种栽培型) 的综合转录组和代谢组分析.
- 采用了高通量测序和液态染色学-并联质谱法 (LC-MS/MS).
- 基因-代谢物相关联网络的构建.
主要成果:
- 培养的菌株显示与 metionin 透酶 MUP1 相关的基因升高,这表明 metionin 代谢发生了变化.
- 代谢分析显示,培养品种的尿素含量较高,野生品种的风味前体 (酸,异氨酸) 含量较高.
- 培养的菌株累积了2-酸和α-甲酸;在6-酸果酸酶和2-酸之间发现了正相关性.
结论:
- 人工栽培显著影响了A. bisporus.中的代谢重编程.
- 特定的基因和代谢物概况区分野生和栽培品种,影响风味和营养价值.
- 这些发现为繁殖A. bisporus提供了关键的分子标,为食用真菌产业提供了增强的特征.
更多相关视频
08:03A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
10.7K
05:29Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Published on: June 9, 2021
4.7K
相关概念视频
Fungal Phylum Basidiomycota
Basidiomycota is a diverse phylum of fungi that includes ecologically significant decomposers such as white rot fungi, symbionts like mycorrhizal fungi, plant pathogens such as rusts and smuts, and edible species like Agaricus bisporus (the common button mushroom). These fungi play crucial roles in nutrient cycling, symbiotic relationships, and even human health. Their defining feature is the basidium, a microscopic club-shaped structure responsible for producing basidiospores.Fruiting Bodies...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Batch vs Continuous Culture
Fermentation is a foundational biotechnological process used to produce pharmaceuticals, biofuels, enzymes, and food additives. Among industrial strategies, batch and continuous fermentation are the two most widely applied. Although both rely on microbial conversion of substrates into desired products, they differ markedly in operation, productivity, and suitability for specific applications.Batch fermentation occurs in a closed system in which nutrient media and inoculum are added at the...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
