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相关概念视频

Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Anticoagulant Drugs: Low-Molecular-Weight Heparins01:30

Anticoagulant Drugs: Low-Molecular-Weight Heparins

Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
Introduction to Hemostasis01:05

Introduction to Hemostasis

Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized, and...
Coagulation01:09

Coagulation

The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...
Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which forms a...
Disorders of Hemostasis01:24

Disorders of Hemostasis

Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.

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相关实验视频

Updated: Jun 17, 2026

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

对于常规凝血试验的共识不稳定方程.

Rubén Gómez Rioja1, Andrea Caballero Garralda2, Immaculada Comas Reixach2

  • 1Hematology Department, La Paz-Cantoblanco-Carlos III Hospital, Madrid, Spain.

Clinical chemistry and laboratory medicine
|March 29, 2025
PubMed
概括
此摘要是机器生成的。

血样本的稳定性对于凝血测试,如前列血时间 (PT) 和激活的部分血栓形成时间 (aPTT) 是至关重要的. 新的共识不稳定方程揭示了降解率,有助于准确的临床实验室测试.

关键词:
凝血是凝固的过程.预分析阶段 预分析阶段样本的稳定性 样本的稳定性稳定性方程中的稳定性方程

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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
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The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

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Published on: February 27, 2026

相关实验视频

Last Updated: Jun 17, 2026

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
10:25

Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro

Published on: March 19, 2016

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

科学领域:

  • 临床化学 临床化学
  • 血液学 血液学 血液学
  • 实验室医学 实验室医学

背景情况:

  • 血样本的稳定性对于前激素时间 (PT) 和激活的部分血栓形成时间 (aPTT) 对于准确的凝血测试至关重要.
  • 临床和实验室标准研究所 (CLSI) 最近的更新延长了aPTT的可接受时间框架,需要更新稳定性数据.
  • 现有的指导方针依赖于具有不同最大允许误差 (MPE) 标准的研究,强调需要采用统一方法.

研究的目的:

  • 通过分析跨越30年的43项研究的原始数据,开发PT和aPTT的共识不稳定方程.
  • 为了描述样品降解,独立于特定的实验参数.
  • 为评估凝血试验稳定性和量化系统性错误提供一个强大的模型.

主要方法:

  • 一项全面的文献综述确定了43项研究,这些研究包含PT,aPTT和关键血凝因子的实验稳定性数据.
  • 分析了包括百分比偏差 (PD%),时间和样本处理变量在内的原始数据.
  • 通过原始回归模型来推导全球不稳定性方程,并确定影响因素.

主要成果:

  • 冷PT和aPTT样本表现出相似的稳定性,平均每月延长0.8%.
  • 在非冷样本中,管道处理对稳定性产生了显著影响,比储存温度更重要.
  • 共识方程表明PT的线性日均恶化为2.9%,aPTT的对数分解,预测分别在6小时和24小时延长6.1%和10%.

结论:

  • 获得的共识不稳定性方程为评估凝血试验稳定性提供了一种可靠的方法.
  • 这些方程与专家建议一致,并增强对样本降解的理解.
  • 这些模型有助于更好地量化凝血测试中的系统错误.