减少乳清蛋白的过敏性,同时提高它们的功能性质和生物活性,使用联合酶
Lidong Pang1, Zhen Huang1, Runze Li1
1Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China.
Food chemistry
|March 29, 2025
概括
结合特定的酶显著降低了乳清蛋白水解剂 (WPH) 的过敏性,并改善了功能性质. 这种双重好处增强了WPH在食品和卫生行业的应用.
科学领域:
- 食品科学 食品科学 食品科学
- 蛋白质化学 蛋白质化学
- 生物技术是生物技术.
背景情况:
- 乳清蛋白水解剂 (WPH) 广泛使用,但可以保持过敏性.
- 酶性水解改变了蛋白质的结构和功能.
- 优化水解条件是提高WPH质量的关键.
研究的目的:
- 研究结合内和外酶对WPH功能性质,生物活性和过敏性的影响.
- 为了确定特定的酶组合如何影响蛋白质构成和水解部位.
- 为了将结构变化与改变的过敏性和改善的功能相关联.
主要方法:
- 使用内酶 (蛋白酶,帕帕因) 和外酶 (ProteAXH) 的组合制备WPH.
- 评估免疫球蛋白E结合能力以测量过敏性.
- 对乳化性能进行评估.
- 对蛋白质构造变化的多谱分析.
- 液体染色学-双重质谱学 (LC-MS/MS) 用于水解部位分析.
- 生物信息学用于表位分析.
主要成果:
- 将蛋白酶或帕帕因与ProteAXH结合,使免疫球蛋白E结合能力分别降低了47.62%和51.91%.
- 在两个酶组合组中,乳化性能大约提高了40%.
- 添加ProteAXH导致蛋白质构成中断的增加和水解部位的改变.
- 降低过敏性与形状破坏和修改的线性表位解部位有关.
- 结构变化促进了乳化和生物活性的释放,增强了功能性质.
结论:
- 使用特定的内皮酶-外皮酶组合的酶化水解有效降低了WPH的过敏性.
- 这些优化的WPH表现出增强的功能性质,包括改进的乳化和生物活性.
- 开发的WPH适用于需要低过敏性和卓越功能性能的应用.
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