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Updated: May 20, 2025

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

Published on: March 17, 2023

根据ATAC-seq和RNA-seq确定肉腹部脂肪中脂肪细胞分化的关键转录因子

Xiaoying Liu¹, Chaohui Wang¹, Xi Sun¹

¹College of Animal Science and Technology, Northwest A&F University, Yangling, PR China.

Poultry science

|March 29, 2025

概括

这项研究揭示了肉腹部脂肪组织 (AFT) 发育的关键分子变化,确定了转录因子和参与脂肪沉积的途径. 结果提供了对表观遗传调节的见解,以改善家禽繁殖策略.

科学领域:

基因组学和分子生物学
禽畜科学与繁殖的研究
表观遗传学和基因调节

背景情况:

密集的 brojler 繁殖导致过度的腹部脂肪组织 (AFT),造成经济损失.
了解AFT分子机制对于开发有针对性的育种策略至关重要.

研究的目的:

为了阐明染色质可访问性和转录性重编程中的动态变化,在 brojler AFT.
确定关键的基因,转录因子和调节不同生长阶段 (D14和D42) AFT发育的途径.

主要方法:

对RNA测序 (RNA-seq) 和ATAC测序 (ATAC-seq) 数据的综合分析.
不同基因表达分析,基因和基因组的京都百科全书 (KEGG) 途径分析和基因组丰富分析 (GSEA).
差异性可访问峰值的分析,DNA动机分析,以及转录和表观基因组数据的整合.

主要成果:

在D14和D42之间观察到显著的转录差异,其中有许多差异表达的基因 (DEG) 和转录因子 (TF).
凯格和GSEA发现了与DNA复制,细胞循环,脂肪酸和类固醇生物合成,自和MAPK信号传递相关的途径的丰富.
ATAC-seq确定了与信号通路 (p53,PPAR,FoxO) 和脂质代谢中的基因相关的差异可访问的峰值. 整合分析突出了ACACA,SCD,SREBF1和KLF9等关键基因的表达变化.
MYB和NFIX被确定为潜在的调控TF,具有脂质代谢基因中的结合位.

结论:

染色质可访问性和基因表达的动态变化是 brojler AFT 发展的基础.
包括MYB和NFIX在内的特定途径和TF在调节脂质代谢和脂肪沉积方面发挥着关键作用.
这些发现为了解肉AFT发育中的表观遗传修饰和转录调节提供了基础,有助于未来的繁殖工作.

关键词:

ATAC-seqq 的使用情况.腹部脂肪在腹部脂肪.脂肪细胞分化的差异化在RNA-seqqq.

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