沃里诺斯塔特通过调节NF-κB和mTOR信号通路来减弱UVB诱导的皮肤衰老
Qianlong Dai1, Zhiwei Wang1, Xue Wang2
1School of Basic Medicine, Dali University, Dali, 671000, Yunnan, China.
Scientific reports
|March 29, 2025
概括
沃里诺斯塔特 (SAHA) 有效地对抗过度紫外线B (UVB) 辐射引起的皮肤衰老. 这项研究表明,SAHA通过抑制mTOR和NF-κB等关键信号通路来缓解细胞衰老和光衰老.
科学领域:
- 皮肤病学 皮肤病学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 过度的紫外线B (UVB) 辐射通过氧化应激和炎症加速皮肤细胞衰老.
- 伏利诺斯塔特 (SAHA) 是一种组织素脱乙酶抑制剂 (HDACi),具有治疗用途,但其在缓解皮肤光衰老方面的作用尚未被探索.
研究的目的:
- 研究SAHA在缓解UVB诱导的皮肤光衰和细胞衰老方面的潜力.
- 阐明SAHA对UVB损伤的保护作用背后的分子机制.
主要方法:
- 在HaCaT细胞和Balb/c小鼠中建立了UVB诱导的光衰老模型.
- 评估了衰老标志物 (β-银酸酶,p16,p21) 和炎症性细胞因子 (IL-1β,IL-6).
- 量化了矩阵金属蛋白酶 (MMP-1,MMP-3,MMP-9) 和分析了mTOR和NF-κB信号通路的激活.
主要成果:
- 紫外线暴露显著上调了HaCaT细胞和小鼠皮肤中的衰老标记物,炎症性细胞因子和MMPs.
- 萨哈补充剂有效地缓解了UVB诱导的细胞衰老和皮肤衰老.
- 紫外线激活了mTOR和NF-κB通路;SAHA治疗抑制了这些通路.
结论:
- 在减轻UVB诱导的皮肤光衰老方面,SAHA显示出显著的潜力.
- 抑制mTOR和NF-κB信号通路是SAHA抗衰老作用的关键机制.
- 萨哈可以作为一种新的治疗剂,用于对抗皮肤光衰老.
相关概念视频
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:31The Intrinsic Apoptotic Pathway
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
01:38Nucleotide Excision Repair
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
02:26NF-kB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:23Role of Skin in Vitamin D Synthesis
The skin plays a crucial role in the synthesis of vitamin D, a vital nutrient for various physiological processes in the body. Vitamin D is unique because it can be synthesized in the skin through a series of chemical reactions triggered by exposure to ultraviolet B (UVB) radiation from sunlight.
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin D3(cholecalciferol).
The solar UV B rays (290-315 nm) are absorbed by the skin, and 7-dehydrocholesterol (provitamin D3) photolyzes it to previtamin D3, which undergoes a rapid transformation to vitamin D3(cholecalciferol).