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Updated: May 7, 2026

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在大肠杆菌中产生来自大脑的神经营养因子和神经营养因子-4在它们的亲和活性形式的协议

Christoph Holzner1, Katharina Böttinger1, Christian G Huber1

  • 1University of Salzburg, Department of Biosciences and Medical Biology, Hellbrunner Straße 34, 5020 Salzburg, Austria; Center for Tumor Biology and Immunology (CTBI), University of Salzburg, 5020 Salzburg, Austria.

STAR protocols
|March 30, 2025
PubMed
概括

这项研究详细介绍了在大肠杆菌中产生脑衍生神经营养因子 (BDNF) 和神经营养素-4 (NT4) 的协议. 该方法涵盖了这些关键的神经营养成长因子的表达,折叠,净化和激活.

关键词:
生物技术和生物工程 生物技术和生物工程蛋白质生物化学 蛋白质生物化学蛋白质表达和净化过程

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Production of Pharmaceuticals01:30

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Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
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