相关实验视频

Updated: Jan 12, 2026

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

Published on: July 10, 2020

土虫的分子反应,Eisenia fetida对氧化 (Benzophenone-3) 暴露的分子反应

Krishna Gautam¹, Sukhveer Singh², Beena Vamadevan³

¹Ecotoxicology Laboratory, Regulatory Toxicology Group, REACT Division, CSIR-Indian Institute of Toxicology Research, C.R. Krishnamurti (CRK) Campus, Lucknow 226008, Uttar Pradesh, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.

The Science of the total environment

|March 30, 2025

概括

索-3 (BP-3) 紫外线过器通过增加氧化应激和改变繁殖和肠道细菌来伤害地. 低水平的长期暴露揭示了对基因表达的非线性影响,影响了陆地生态系统.

科学领域:

环境毒理学环境毒理学
生态毒理学生态毒理学
土壤科学土壤科学

背景情况:

索类型的紫外线过器,如索-3 (BP-3),正在审查潜在的人类和环境毒性.
有机紫外线过器可以在环境中持续存在,这引发了人们对其对非目标生物的影响的担忧.

研究的目的:

为了研究索-3 (BP-3) 对土虫Eisenia fetida的毒理作用.
在关键的陆地无脊椎动物模型中阐明了BP-3毒性背后的分子机制.

主要方法:

暴露Eisenia fetida对BP-3的不同度的暴露.
全球转录组学 (KEGG和GO分析) 以确定差异表达的基因.
定量PCR (q-PCR) 用于基因表达的验证.
分析土壤微生物群落结构 (细菌和真菌多样性).

主要成果:

长期,低水平的BP-3暴露增加了活性氧物种 (ROS),并对的生殖器官和整体生殖产生了负面影响.
转录组分析显示,参与应激反应 (CuZn-SOD,CAT),新陈代谢 (GST),生殖 (ANN,Piwi-2) 和内分泌功能 (EcR) 的基因显著上调.
暴露于BP-3改变了肠道微生物群中的细菌多样性,蛋白质细菌,菌细菌,动菌细菌和细菌类细菌发生了显著的变化,而真菌多样性基本上没有受到影响.

结论:

暴露于BP-3会诱导地的分子和生理干扰,影响压力,繁殖和内分泌功能.
该研究强调了BP-3对特定基因表达的非线性剂量反应关系,特别是在生殖和内分泌途径中.
BP-3显著改变了土壤细菌群落,表明了对陆地生态系统的潜在风险以及土壤生态毒理学中化学风险评估的需要.

关键词:

索芬-3的使用方法失生症是一种失生症.土虫土虫是一种虫.生殖生殖的繁殖转录组转录组就是一个转录组.

更多相关视频

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Published on: February 22, 2018

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

Published on: December 4, 2017

相关概念视频

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

The Mechanics of (Poro-)Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

In this work, an in vitro reconstitution approach is employed to study the poroelasticity of actomyosin gels under controlled conditions. The dynamics of the actomyosin gel and the embedded solvent are quantified, through which the network poroelasticity is demonstrated. We also discuss the experimental challenges, common pitfalls, and relevance to cell cytoskeleton...

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

Macro-Rheology Characterization of Gill Raker Mucus in the Silver Carp, Hypophthalmichthys molitrix

This protocol presents a method to perform rheology characterization of mucus that resides on gill rakers (GRs) of the silver carp. Viscoelastic characteristics of GR-mucus, obtained by measuring viscosity, storage and loss moduli, are evaluated for the apparent yield stress to understand the filter feeding mechanism in...

The Diffusion of Passive Tracers in Laminar Shear Flow

The Diffusion of Passive Tracers in Laminar Shear Flow

A protocol for the study of the diffusion of passive tracers in laminar pressure-driven flow is presented. The procedure is applicable to various capillary pipe...

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Visually Based Characterization of the Incipient Particle Motion in Regular Substrates: From Laminar to Turbulent Conditions

Two different methods for characterizing the incipient particle motion of a single bead as a function of the sediment bed geometry from laminar to turbulent flow are...

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

An Analog Macroscopic Technique for Studying Molecular Hydrodynamic Processes in Dense Gases and Liquids

An experimentally accessible analog method for studying molecular hydrodynamic processes in dense fluids is presented. The technique uses particle image velocimetry of vibrated, high-restitution grain piles and allows direct, macroscopic observation of dynamical processes known and predicted to exist in strongly interacting, high density gases and...

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Confocal microscopy is used to image quiescent and flowing colloid-polymer mixtures, which are studied as model systems for attractive suspensions. Image analysis algorithms are used to calculate structural and dynamic metrics for the colloidal particles that measure changes due to geometric...