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铁诱导的酶,尼古丁胺合成酶的特征,来自巨型白树

James T Carrillo¹, Dulal Borthakur¹

¹University of Hawaii at Manoa, Department of Molecular Biosciences and Bioengineering, 1955 East-West Road, Agricultural Sciences 218, Honolulu, HI 96822, United States.

Plant science : an international journal of experimental plant biology

|March 30, 2025

概括

巨型花植物通过改变基因表达,特别是尼古丁胺胺合成酶来调节铁的稳态. 试验室研究表明,尼古丁胺胺合成酶活性由特定的酶增强,但无细胞生产尚未在工业上可行.

科学领域:

植物生物学植物生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

巨型白树 (Leucaena leucocephala亚种) 是一个巨型白树. 玻璃) 产生mimosine,一种铁化化合物,使其金属平衡至关重要.
了解植物的铁吸收和新陈代谢对于农业和植物疗法至关重要.

研究的目的:

为了研究巨型树中铁恒温的分子机制.
描述尼古丁胺胺合成酶的特征,并优化其体外活性.

主要方法:

实时PCR分析铁应用后的基因表达变化.
转录组测序和5'-RLM-RACE用于识别基因序列.
对尼古丁胺胺合成酶和相关酶的异质表达,净化和体外酶定量.

主要成果:

外源性铁的应用上调了19个基因,包括谷氨合成酶,铁酸还原酶, μιμοσινάση和尼古提安胺合成酶.
确定了巨型白树尼可提安胺合成酶的完整编码序列.
实验室试验表明,尼古胺合成酶的活性高度依赖于5'-甲基氨酸核酶,对S-氨酸氨酸 (SAM) 纯度敏感,在现场SAM生产增加了活性五倍.

结论:

巨型白中铁的稳定性涉及复杂的基因调节,尼古丁胺胺合成酶发挥着关键作用.
在体外优化尼古丁胺胺合成酶活性需要特定的辅助酶和纯基质.
目前的无细胞生产方法尚未适用于工业应用,但对酶机制的进一步研究具有前景.

关键词:

不同类的表达式不同类的表达式金属化化金属化植物光体 (Phytosiderophores) 是一种植物光体.植物多氨基酸是植物多氨基酸.再折叠可以重新折叠.取决于SAM的酶.

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