基于益生菌的免疫改善了Oreochromis mossambicus对Aeromonas hydrophila病原体的免疫反应:来自Bacillus licheniformis (Dahb1) 的见解
Jeyaraj Jeyavani1, Narayanan Gopi1, Khalid A Al-Ghanim2
1Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Alagappa University, Karaikudi, 630003, Tamil Nadu, India.
Microbial pathogenesis
|March 30, 2025
概括
益生菌Bacillus licheniformis的细胞成分,特别是细胞壁蛋白 (CWP),增强了鱼 (Oreochromis mossambicus) 的免疫反应. 这些成分提供了对Aeromonas hydrophila的保护,改善了鱼类的存活率,并控制了细菌感染.
科学领域:
- 水产养殖免疫学 水产养殖免疫学
- 鱼类疾病耐药性 鱼类疾病耐药性
- 益生菌在水产养殖中的应用.
背景情况:
- 水 (Aeromonas hydrophila) 对水产养殖业构成重大威胁,造成了相当大的经济损失.
- 益生菌为预防鱼类疾病提供了抗生素的可持续替代品.
- 了解益生菌的特定免疫调节成分对于有效的疾病管理至关重要.
研究的目的:
- 为了评估来自Oreochromis mossambicus中的Bacillus licheniformis的全细胞蛋白 (WCP),细胞外蛋白 (ECP) 和细胞壁蛋白 (CWP) 的免疫潜力.
- 评估这些成分对Aeromonas hydrophila感染的疗效.
- 研究免疫增强的潜在机制.
主要方法:
- 奥里奥克罗米斯·莫桑比克斯 (Oreochromis mossambicus) 接种了Bacillus licheniformis的WCP,ECP和CWP的免疫.
- 免疫接种后14天和28天测量了幽默和细胞免疫参数.
- 鱼被挑战了Aeromonas hydrophila,并记录了生存率.
- 评估了细胞组件的体外抗菌和抗生物膜活性.
主要成果:
- 用CWP,WCP和ECP接种疫苗显著增加了幽默和细胞免疫参数.
- 观察到高反应性氧物种 (ROS),超氧化物和基离子,增强免疫活性.
- 在A. hydrophila挑战后,在用益生菌成分免疫的鱼类中观察到生存率的显著增加.
- 隔离的细胞成分显示出强大的抗菌和抗生物膜活性,对抗A. hydrophila.
结论:
- 益生菌Bacillus licheniformis的细胞成分,特别是CWP,有效地刺激了O. mossambicus的免疫反应.
- 这些成分对鱼的Aeromonas hydrophila感染提供了显著的保护.
- 细菌菌的细胞成分代表了控制水产养殖中的细菌疾病的有希望的战略.
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