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Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis07:45

Draining Lymph Node Metastasis Model for Assessing the Dynamics of Antigen-Specific CD8+ T Cells During Tumorigenesis

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The experimental design presented here provides a useful reproductive model for the studies of antigen-specific CD8+ T cells during lymph node (LN) metastasis, which excludes the perturbation of bystander CD8+ T...
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A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice09:17

A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice

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Clonal expansion is a key feature of antigen-specific T cell response. However, the cell cycle of antigen-responding T cells has been poorly investigated, partly because of technical limitations. We describe a flow cytometric method to analyze clonally expanding antigen-specific CD8 T cells in spleen and lymph nodes of vaccinated...
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Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

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We describe here a flow cytometry-based in vivo killing assay that enables examination of immunodominance in cytotoxic T lymphocyte (CTL) responses to a model tumor antigen. We provide examples of how this elegant assay may be employed for mechanistic studies and for drug efficacy...
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In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues08:37

In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

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Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.
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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining06:25

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Here, we present an optimized protocol for enumerating and characterizing rhesus macaque CD8+ T cells against the AIDS virus. This article is useful not only to the field of HIV immunology, but also to other areas of biomedical research where CD8+ T cell responses are known to affect disease...
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Examination of Thymic Positive and Negative Selection by Flow Cytometry14:29

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We present a flow cytometry-based method to examine T cell development in vivo using genetically manipulated mice on a wildtype or T cell receptor transgenic...
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相关实验视频

Updated: Feb 3, 2026

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
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在RISPERIDONE加载的PLGA微球中,衰老诱导的微结构演变.

Andrew G Clark1, Jeffrey Wong1, Ruifeng Wang2

  • 1DigiM Solution LLC, 500 West Cummings Park Suite 3650, Woburn, MA, the United States of America.

International journal of pharmaceutics
|March 30, 2025
PubMed
概括
此摘要是机器生成的。

老化聚 (乳糖-糖酸) 微球增加毛孔和毛孔大小,影响药物释放. 这项研究使用先进的成像来量化结构变化,揭示了聚合物放松对治疗性能的影响.

关键词:
有控制释放的释放.基于图像的真实密度表征.微观结构量化的量化.在 PLGA PLGA 中.产品质量产品质量 产品质量

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Examination of Thymic Positive and Negative Selection by Flow Cytometry
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Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining
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科学领域:

  • 材料科学 材料科学 材料科学
  • 制药科学 制药科学
  • 聚合物化学 聚合物化学

背景情况:

  • 聚合物老化可能会影响药物输送系统的性能.
  • 了解由于衰老而导致的聚 (乳糖-糖酸) (PLGA) 微球的结构变化对于治疗疗效至关重要.
  • 微球的结构完整性对于可预测的药物释放概况至关重要.

研究的目的:

  • 用先进的成像技术来量化PLGA微球中的结构变化作为衰老的函数.
  • 将老化和新鲜的微球批量进行比较,以了解老化对关键质量属性 (CQA) 的影响.
  • 为了将观察到的结构变化与体外药物释放性能相关联.

主要方法:

  • 相关聚焦离子束扫描电子显微镜 (FIB-SEM) 和X射线显微镜 (XRM) 用于纳米和批量级结构特征.
  • 开发了一种基于XRM的新方法来确定材料密度.
  • 分析了老化 (过了一年的保质期) 和新鲜的微球批量.

主要成果:

  • 衰老导致纳米尺度上的毛孔和毛孔大小增加,这归因于PLGA的物理放松.
  • 在老化的微球批量中观察到物质密度的下降.
  • 增加的多孔度与改变的体外药物释放性能相关.

结论:

  • 在PLGA微球中聚合物老化会通过放松增加毛孔度,扩大现有的毛孔.
  • 先进的成像技术有效量化老化引起的结构变化.
  • 这项研究提供了一种新的方法来评估聚合物衰老对PLGA微球药物递送系统的影响.