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相关概念视频

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Gastrulation01:56

Gastrulation

Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

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相关实验视频

Updated: May 12, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

在小鼠胚胎中胃化过程中的替代拼接动态.

Wei Wang1, Yu Zhang1, Yuanyuan Zhai1

  • 1Inner Mongolia Key Laboratory of Life Health and Bioinformatics, School of Life Science and Technology, Inner Mongolia University of Science and Technology, Baotou, China.

Scientific reports
|March 30, 2025
PubMed
概括

替代拼接 (AS) 动态和拼接因子 (SF) 表达在小鼠胚胎胃化过程中至关重要. 这项研究揭示了AS关键事件和调节机制在发育的胚胎层.

关键词:
另一个替代拼接方法.胚胎发育的时间表观遗传调节 表观遗传调节鼠标的胃化是鼠标的胃化拼接因素 拼接因素

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

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Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation
09:14

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

Published on: June 14, 2024

相关实验视频

Last Updated: May 12, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation
09:14

Single-Cell RNA Sequencing of Mutant Whole Mouse Embryos: From the Epiblast to the End of Gastrulation

Published on: June 14, 2024

科学领域:

  • 发展生物学 发展生物学
  • 分子生物学分子生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

  • 替代拼接 (AS) 对发育,分化和癌症至关重要.
  • 在小鼠胃化过程中拼接调节的机制尚未完全理解.
  • 胃流形成了哺乳动物胚胎中的三个主要生殖层.

研究的目的:

  • 研究小鼠胃化过程中替代拼接的动态 (E6.5-E7.5).
  • 揭示AS在初级生殖层中的调节机制.
  • 描述胃流动过程中拼接因子 (SFs) 的表达模式和功能.

主要方法:

  • 空间时间转录组和表观组数据的分析.
  • 检测和描述AS事件和差异替代拼接事件 (DASEs).
  • 拼接因子 (SF) 表达和相关的表观遗传信号的分析.

主要成果:

  • AS和DASEs表现出动态变化,在晚期胃流动中显著增加.
  • SFs表现出特定阶段的表达,在中期和晚期胃流动中呈高水平.
  • 与SF和AS部位相关的表观遗传信号在胃流动过程中得到丰富和动态变化.

结论:

  • 这项研究提供了在小鼠胃流动过程中对AS的系统分析.
  • 识别了初级生殖层特定的AS事件,并描述了SF表达和表观遗传调节.
  • 增强对生殖层形成机制的理解,重点关注前mRNA AS.