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裸眼现场检测多种传染病,使用等离子增强光纳米粒子

Xuan Tan¹, Jingkai Yang¹, Ying Liu¹

¹Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen 518055, China.

Analytical chemistry

|March 31, 2025

概括

一种新型的等离子银膜增强可见光的光,用于检测传染病. 这种方法允许用肉眼诊断C型肝炎病毒 (HCV),B型肝炎病毒 (HBV) 和人类免疫缺陷病毒 (HIV) 抗体.

科学领域:

纳米技术纳米技术
生物医学诊断生物医学诊断
传染病检测检测传染病检测

背景情况:

在资源有限的环境中,准确的传染病诊断具有挑战性.
等离子材料通过局部表面等离子共振 (LSPR) 提高光检测灵敏度.
传统的方法往往需要过器裸眼观察由于小斯托克斯移动.

研究的目的:

开发一种新的等离子银膜 (pSilverF),用于增强可见光光检测.
为了能够用肉眼观察生物标志物检测,提高灵敏度.
创建一个多重测试,用于检测C型肝炎病毒 (HCV),B型肝炎病毒 (HBV) 和人类免疫缺陷病毒 (HIV) 抗体.

主要方法:

制造一种新的等离子银膜 (pSilverF).
集成pSilverF与光纳米粒子进行多重检测.
使用开发的平台检测HCV,HBV和HIV的抗体.
使用RGB图像从标准手机摄像头图像中分割的结果的分析.

主要成果:

获得HCV,HBV和HIV抗体的高检测灵敏度 (分别降至0.0032,0.023和0.168NCU/mL).
在临床样本中证明了100%的HIV和HCV检测灵敏度和特异性.
在临床样本中表现出96%的敏感性和100%的HBV检测特异性.
启用裸眼可视化和手机摄像头捕捉结果,没有过器.

结论:

pSilverF平台显著增强可见光光,用于敏感的生物标志物检测.
开发的多重检测方法为诊断多种传染病提供了一种简单,无过的方法.
这项技术有可能在资源有限的环境中进行基于现场的传染病查.

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Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.