凯姆菲罗尔向全球表观遗传修饰剂,阻碍HeLa细胞的生长和迁移能力
Nazia Afroze1, Shafiul Haque2,3, Arif Hussain1
1School of Life Sciences, Manipal Academy of Higher Education, Dubai, UAE.
Journal of cellular and molecular medicine
|March 31, 2025
概括
凯姆费罗尔是一种饮食化合物,通过改变HeLa细胞中的DNA甲基化和基因素标记来修改表观基因组. 这种表观遗传重编程会重新激活瘤抑制基因,抑制癌细胞的生长和迁移.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 癌症生物学 癌症生物学
- 营养生物化学 营养生物化学
背景情况:
- 饮食中的生物活性剂通过影响染色素,显示出在癌症治疗中的潜力.
- 表观遗传修饰在瘤的进展和发育中起着至关重要的作用.
研究的目的:
- 为了研究kaempferol对HeLa细胞的表观遗传作用.
- 阐明kaempferol影响基因表达和细胞行为的机制.
主要方法:
- 定量PCR (qPCR) 用于基因表达分析.
- 素修饰量化的ELISA测定.
- 用于促进体甲基化分析的DNA甲基序列测序.
- 基于细胞的测试 (殖民地形成,入侵,伤) 用于功能评估.
主要成果:
- 凯姆菲罗尔降低了DNA甲基转移酶 (DNMTs) 和基因素脱乙酶 (HDACs) 的表达和活性.
- 基因素H3和H4的修饰是由kaempferol调节的.
- 凯姆菲罗尔诱导了瘤抑制基因 (TSG) 的低甲基化和重新激活,如WIF1,RUNX1,RARβ和SOX1.
- 凯姆菲罗尔对HeLa细胞表现出显著的增长抑制和抗迁移作用.
结论:
- 凯姆菲罗尔作为癌细胞中的表观遗传修饰剂.
- 它的机制涉及DNA甲基化和基因组修饰的调节,导致TSG的重新激活.
- 凯姆菲罗尔通过表观遗传重编程表现出作为抗癌剂的潜力.
更多相关视频
10:28Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
09:56Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
Published on: October 31, 2025
相关概念视频
Epigenetic Regulation
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Epigenetic Regulation
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
