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聚烯微型和纳米塑料暴露于模拟的人类消化酶系统:结构和功能影响

Ananthaselvam Azhagesan¹, Natarajan Chandrasekaran¹

¹Centre for Nanobiotechnology, Vellore Institute of Technology (VIT), Vellore, Tamil Nadu 632014, India.

|March 31, 2025

概括

聚乙烯纳米塑料 (PS-NP) 通过改变它们的结构和活性来破坏像粉酶和素这样的消化酶. 这项研究揭示了摄入这些微塑料和纳米塑料的潜在健康风险.

科学领域:

环境科学环境科学
生物化学生物化学
毒理学毒理学毒理学

背景情况:

新出现的污染物,聚烯微型和纳米塑料 (PS-MNPs),越来越多地被摄入.
了解它们与人类消化酶的相互作用对于评估健康风险至关重要.
之前的估计表明,每天摄入的微塑料量显著.

研究的目的:

为了研究PS-MNP与关键消化酶的体外相互作用.
阐明由PS-MNP抑制酶的基础分子机制.
评估PS-MNP暴露对消化酶功能的潜在健康影响.

主要方法:

消化酶 (α-氨酶,素,胰腺素) 对PS MPs和PS NPs的暴露.
使用的光谱技术:光发射,共振光散射 (RLS),里埃变换红外线 (FT-IR) 和循环二元化 (CD).
确定酶抑制动力学 (IC50值) 和分析结构变化.

主要成果:

PS NPs引起了显著的结构变化和消化酶的抑制,比PS MPs更多.
光谱学表明静态火,影响了氨酸和氨酸残留物.
RLS证实了酶-PS NP复合物的形成和聚合;FT-IR和CD显示蛋白质含量降低和酶结构改变.
PS NPs表现出各种消化酶的非竞争性和混合可逆抑制.

结论:

聚乙烯纳米塑料通过抑制和结构性改变关键的消化酶,构成潜在的健康风险.
这些发现为塑料污染物和人类生物系统之间的分子相互作用提供了洞察力.
需要进一步的研究,才能充分了解摄入PS-MNP的长期健康后果.

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