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Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

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Updated: May 5, 2026

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

通过计算式三光子显微镜进行深度结构性脑成像.

Lingmei Chen¹, Mubin He¹, Lu Yang¹

¹Zhejiang University, College of Optical Science and Engineering, International Research Center for Advanced Photonics, State Key Laboratory of Extreme Photonics and Instrumentation, Hangzhou, China.

Journal of biomedical optics

|March 31, 2025

概括

此摘要是机器生成的。

我们开发了低级扩散模型三光子显微镜 (LRDM-3PM) 用于深层组织成像. 这种方法可以在1.5毫米深度的活老鼠大脑中获得高分辨率图像,克服分散的挑战.

关键词:

大脑成像 - - 大脑成像大脑血管系统的形成深度学习是一种深度学习.形态特征形态特征形态特征三光子显微镜三光子显微镜

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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Published on: January 13, 2022

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Published on: January 17, 2025

相关实验视频

Last Updated: May 5, 2026

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices

Published on: July 26, 2011

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Published on: January 13, 2022

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses

Published on: January 17, 2025

科学领域:

生物医学光学生物医学光学
神经成像是一种神经成像.
计算成像技术的成像

背景情况:

生物组织中的光学成像受限于在相当深处的光散射.
现有的方法在复杂的生物结构中难以保持图像质量.

研究的目的:

开发一种计算深度三光子显微镜 (3PM) 方法,用于增强深层组织成像.
为了提高图像质量而不牺牲速度,增加功率或添加光学组件.

主要方法:

引入了低级扩散模型-三光子显微镜 (LRDM-3PM).
利用聚合诱导的发射纳米探测器和自我监督的深度学习.
利用表面图像信息来补偿散射和系统噪声.

主要成果:

在1.5毫米深度实现的信号到背景比>100.
在活小鼠大脑中启用海马的成像.
与用于大脑血管形态结构分析的3D多参数分析平台集成.

结论:

LRDM-3PM 促进了微侵袭性体内成像和分析.
代表了深层组织成像技术的重大进步.
在前所未有的深度保持高分辨率的成像质量.