评估储存和评估呼吸器毛囊运动性的液体介质要求
1College of Medicine and Dentistry, James Cook University, Townsville, QLD, Australia.
PeerJ
|March 31, 2025
概括
媒介的选择显著影响呼吸器毛囊的运动性. 盐水会迅速损害功能,而其他介质可保持长达12小时的运动性,特别是在4°C保存时. 最佳的介质选择是准确的乳毛节拍频率研究的关键.
科学领域:
- 细胞生物学 细胞生物学
- 呼吸系统生理学 呼吸系统生理学
背景情况:
- 粘膜干净对于肺部健康至关重要.
- 呼吸驱动粘膜干净,其功能通过拍数频率 (CBF) 进行评估.
- 报告的正常CBF值的变化可能源于研究中使用的各种介质.
研究的目的:
- 为了研究不同的液体介质如何影响呼吸的运动性.
- 为了确定储存时间和温度对各种介质中的乳毛功能的影响.
主要方法:
- 使用了成年C57/BL6小鼠气管样本.
- 样品在八种介质中进行化:盐水,DPBS,HBSS,M199,DMEM,RPMI,MEM和L-15,有或没有10%的FBS.
- 评估了储存时间 (0-12小时) 和温度 (4°C或室温) 对毛运动性的影响.
主要成果:
- 除盐水以外的所有介质在新成像样本中保持了乳毛功能.
- 盐水在储存一个小时后显著减少了运动毛.
- 在4°C的储存中,保存的乳毛功能比室温更好,这增加了CBF,特别是FBS.
- 在L-15介质中,FBS对于维持乳毛运动是必不可少的.
结论:
- 盐水不适合存储超出直接成像的状样本.
- 大多数常见的介质在4°C储存时同样保持毛功能长达12小时.
- DPBS是一种成本效益高的替代品,用于保存状样本.
- 储存室温可以增加CBF,特别是在含有FBS的介质中.
- L-15介质需要FBS补充剂来维持乳毛运动.
更多相关视频
09:03Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic
Published on: November 7, 2020
4.6K
07:40Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium
Published on: February 21, 2025
425
相关概念视频
01:17Assessment of Diffusion and Perfusion
Understanding and evaluating diffusion and perfusion is critical in assessing a patient's respiratory and circulatory health. These processes play key roles in maintaining the body's internal environment, ensuring that tissues receive adequate oxygen while waste products are efficiently removed.
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this principle...
The Role of Diffusion in Respiration
Diffusion is the process by which molecules move from an area of higher concentration to an area of lower concentration. In the respiratory system, this principle...
01:23Assessment of Respiration
The respiratory system's basic structures and primary functions lay the foundation for nurses' comprehensive respiratory assessments. This assessment includes subjective and objective data to gauge the patient's respiratory health.
Subjective Assessment: Nurses interview the patient to gather information directly during the subjective assessment. It includes questions about the individual's medical history, medications, and symptoms, focusing on past respiratory conditions like asthma or COPD,...
Subjective Assessment: Nurses interview the patient to gather information directly during the subjective assessment. It includes questions about the individual's medical history, medications, and symptoms, focusing on past respiratory conditions like asthma or COPD,...
01:19Respiratory Assessment: Purpose and Indications
Respiratory assessment is a cornerstone of nursing assessments, crucial for the early detection of patient deterioration. This evaluation transcends routine procedures, representing a critical skill nurses must master to ensure optimal patient care.
Objectives and Importance:
The primary goal of respiratory assessment is to evaluate patients at early risk of clinical deterioration. Since respiratory distress often precedes other signs of declining health, breathing patterns and sounds become a...
Objectives and Importance:
The primary goal of respiratory assessment is to evaluate patients at early risk of clinical deterioration. Since respiratory distress often precedes other signs of declining health, breathing patterns and sounds become a...
01:30Assessment of Airway, Skin Color, and Use of Accessory Muscles
A thorough assessment of respiratory health is paramount in clinical settings to identify and manage respiratory distress and ensure adequate oxygenation. This article elaborates on the critical aspects of respiratory evaluation, including airway assessment, skin color examination, and the observation of accessory muscle use, which are integral to effectively diagnosing and managing patients with respiratory conditions.
Introduction
The initial evaluation of a patient's respiratory system...
Introduction
The initial evaluation of a patient's respiratory system...
01:29Assessment of Ventilation II: Respiratory Depth and Rhythm
Respiratory Depth
Respiratory depth measures the volume of air inhaled or exhaled during a breath. It can vary from shallow to deep and typically remains consistent when a person is at rest or asleep. Occasionally, individuals will automatically inhale deeply, known as sighing, which inflates the lungs with more air than normal breathing.
To assess respiratory depth, observe the degree of chest excursion or movement:
Respiratory depth measures the volume of air inhaled or exhaled during a breath. It can vary from shallow to deep and typically remains consistent when a person is at rest or asleep. Occasionally, individuals will automatically inhale deeply, known as sighing, which inflates the lungs with more air than normal breathing.
To assess respiratory depth, observe the degree of chest excursion or movement:
01:27Physical Assessment of the Respiratory Tract II: Inspection
Physical assessment of the respiratory tract through inspection is a crucial step in understanding the patient's respiratory health. It provides insights into the functioning of the respiratory system, the musculoskeletal structure, and even the patient's nutritional status. This comprehensive approach involves observing several vital aspects: chest configuration, breathing patterns, respiratory rates, skin color, and use of accessory muscles.
Chest Configuration
The chest configuration can...
Chest Configuration
The chest configuration can...