复制性自私的遗传元素正在推动Enterococcus faecium的快速病原性适应
Matthew P Grieshop1,2,3, Aaron A Behr4, Sierra Bowden1
1Department of Genetics, Stanford University, Stanford, CA, USA.
bioRxiv : the preprint server for biology
|March 31, 2025
概括
插入序列 (IS) 驱动了医疗保健病原体的快速基因组进化,例如Enterococcus faecium. ISL3家族活跃复制,导致结构变异和增强基因表达,以改善临床环境中的生存率.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 进化生物学 进化生物学
背景情况:
- 与医疗保健相关的病原体构成了相当大的负担.
- 了解病原体适应对于控制策略至关重要.
研究的目的:
- 研究插入序列 (IS) 作为医疗保健病原体中快速基因组进化的媒介.
- 确定ISL3家族元素在Enterococcus faecium适应中的作用.
主要方法:
- 对28,207个公共病原体基因组的分析.
- 282个Enterococcus faecium临床分离物的长读测序.
- 从血造细胞移植患者的28个纵向便样本的长读测序.
- 对ISL3活动及其监管效应的基因组和转录组分析.
主要成果:
- 在E. faecium,Streptococcus pneumoniae和Staphylococcus aureus中发现的复制ISL3家族的高副本数量.
- 在过去的30年里,ISL3的扩散在E. faecium中有所增加.
- 通过活性ISL3元素介导的E. faecium基因组的广泛结构变异.
- 在临床时间尺度内观察到ISL3复制和运动在肠道微生物群中.
- 插入ISL3增强了folT基因表达,改善了E. faecium中的叶酸清理.
结论:
- 在医疗保健相关的E. faecium中,ISL3扩张是快速基因组进化的主要驱动因素.
- 活跃的ISL3复制有助于E. faecium在临床环境中的代谢适应性和适应性.
- 这种基因组可塑性影响了E. faecium在医疗保健环境中的进化轨迹.
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