相关实验视频
Updated: May 17, 2025

10:36
Measurements of Physiological Stress Responses in C. Elegans
Published on: May 21, 2020
13.8K
压力依赖的PQM-1激活调节了生物生存的第二波蛋白质稳定反应
Laura Jones1, Valeria Uvarova2, Daniel O'Brien1
1School of Molecular and Cell Biology & Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, United Kingdom.
bioRxiv : the preprint server for biology
|March 31, 2025
概括
转录因子PQM-1通过调节蛋白质质量,对生存热和氧化应激至关重要. 它的激活取决于DAF-16信号,但饮食限制允许PQM-1独立运作,增强应激弹性.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 细胞生物学 细胞生物学
背景情况:
- 蛋白质稳定性转录因子对于管理细胞应激和维持蛋白质稳定性至关重要.
- 指转录因子PQM-1在各种压力条件下对生物的生存起着至关重要的作用.
研究的目的:
- 阐明控制压力反应期间PQM-1活动的调节机制.
- 研究PQM-1在不同环境条件下的抗压能力和蛋白质质量控制中的作用.
主要方法:
- 研究了通过ILS-DAF-16信号和酸化在Threonine 268的PQM-1调节.
- 利用转录和生物信息分析来识别PQM-1的基因.
- 在充分养和饮食限制条件下检查了PQM-1局部化和功能.
主要成果:
- 在养良好的成年人中,PQM-1的激活依赖于DAF-16在热冲击后恢复期间的信号传递.
- 饮食限制绕过了DAF-16的依赖,使PQM-1能够独立地赋予应激弹性.
- PQM-1对于提高压力反应基因的调节至关重要,有助于生物体的恢复和生存.
结论:
- PQM-1激活集成了环境线索,如饮食限制和ILS-DAF-16信号,以确保蛋白质稳定.
- 在压力恢复过程中,PQM-1调节了一组独特的基因,可能调解二次应激反应.
- 这项研究揭示了一种对压力依赖的PQM-1激活的新机制,这对于生物的生存至关重要.
相关概念视频
The Unfolded Protein Response
4.3K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
4.3K
Regulation of the Unfolded Protein Response
2.3K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.3K
Abnormal Proliferation
4.4K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.4K
The Proteasome
783
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
783
DNA Damage can Stall the Cell Cycle
9.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.0K
Regulated Protein Degradation
7.1K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.1K

