Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Alternative RNA Splicing02:18

Alternative RNA Splicing

20.9K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.9K
RNA Splicing01:32

RNA Splicing

55.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
55.8K
Mutations01:39

Mutations

77.5K
Overview
77.5K
Types of RNA01:20

Types of RNA

5.5K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.5K
RNA Stability01:53

RNA Stability

33.1K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.1K
Translation01:31

Translation

14.2K
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are...
14.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

PRMT5 activity sustains histone production to maintain genome integrity.

bioRxiv : the preprint server for biology·2025
Same author

Finding and recycling stalled spliceosomes.

Nature structural & molecular biology·2025
Same author

Smoking-Associated Carcinogen-Induced Inflammation Promotes Lung Carcinogenesis via IRAK4 Activation.

Clinical cancer research : an official journal of the American Association for Cancer Research·2024
Same author

Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation.

eLife·2022
Same journal

Layered social competition coordinates reproductive hierarchy formation in ants.

bioRxiv : the preprint server for biology·2026
Same journal

Combination epigenetic-targeted therapy increases the immunogenicity of poorly immunogenic sarcomas.

bioRxiv : the preprint server for biology·2026
Same journal

Loss of LanC-like proteins delays post-injury regeneration of aging skeletal muscles.

bioRxiv : the preprint server for biology·2026
Same journal

Integrative Transfer Network: Deep Transfer Learning Across Populations and Prediction Targets.

bioRxiv : the preprint server for biology·2026
Same journal

Confidence-supported label-free metabolic imaging with FPhaS phase autofluorescence microscopy.

bioRxiv : the preprint server for biology·2026
Same journal

Sequence-encoded autoinhibition couples mRNA decapping activity to phase separation.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: May 17, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

2.6K

U2-2 snRNA 突变改变了转录基因组.

Zhongxuan Chi, Varun Gupta, Charles Query

    bioRxiv : the preprint server for biology
    |March 31, 2025
    PubMed
    概括
    此摘要是机器生成的。

    在U2-2小核RNA (snRNA) 中的突变会影响基因表达和拼接. 降低的U2-2水平,就像淘汰细胞一样,显著改变了转录组,影响了RNA处理途径.

    更多相关视频

    ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
    07:31

    ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

    Published on: June 30, 2022

    2.4K
    Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
    11:34

    Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

    Published on: August 9, 2019

    6.6K

    相关实验视频

    Last Updated: May 17, 2025

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
    08:53

    A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

    Published on: September 15, 2021

    2.6K
    ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
    07:31

    ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

    Published on: June 30, 2022

    2.4K
    Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
    11:34

    Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

    Published on: August 9, 2019

    6.6K

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 在RNA生物学,RNA生物学.

    背景情况:

    • 结合体,对于从前mRNA中移除内子至关重要,由含有snRNA的snRNP组成.
    • U2 snRNA在通过RNA-RNA和RNA-蛋白相互作用的拼接中发挥着至关重要的作用.
    • 在U2 snRNA中发生的突变,特别是在C28中,与癌症的发展有关.

    研究的目的:

    • 为了研究由U2-2 snRNA突变引起的基因表达改变.
    • 为了比较U2-2突变与U2-2水平对拼接和基因表达的影响.

    主要方法:

    • 构建U2-2C28突变,淘汰赛 (KO) 和过度表达 (OE) 细胞系.
    • RNA测序以分析全球基因表达变化.
    • 拼接模式和差异表达基因的分析.

    主要成果:

    • 淘汰U2-2 snRNA导致了显著的拼接变化,并改变了4000多个基因的表达.
    • 与RNA处理和非编码RNA相关的基因表达途径显著丰富.
    • 拼接模式对U2-2剂量比仅对突变更敏感.

    结论:

    • U2-2 snRNA突变和改变的水平显著影响拼接和转录组.
    • U2-2淘汰赛在很大程度上模仿了在U2-2突变体中观察到的拼接变化.
    • 无论是U2-2水平还是特定突变,都会导致变化的拼接,许多事件可能导致无意中介衰变 (NMD).