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Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

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相关实验视频

Updated: Jun 28, 2026

A Protocol for Decellularizing Mouse Cochleae for Inner Ear Tissue Engineering
09:53

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关于软骨脱细胞化的技术方法的最新情况:基于物理的协议.

Hengameh Dortaj1,2, Ahmad Vaez1, Ashraf Hassanpour-Dehnavie3

  • 1Department of Tissue Engineering and Applied Cell Sciences, School of Advance Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

BioImpacts : BI
|March 31, 2025
PubMed
概括

这项研究开发了一种用于软骨细胞外基质 (ECM) 支架的新型物理脱细胞化方法. 优化的协议有效地去除细胞,同时保持ECM结构,显示了软骨组织工程没有有毒化学物质的希望.

关键词:
软骨 软骨是一种软骨.细胞外矩阵是细胞外矩阵.物理脱细胞化 物理脱细胞化组织工程是组织工程.

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相关实验视频

Last Updated: Jun 28, 2026

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科学领域:

  • 生物材料科学 生物材料科学
  • 组织工程是组织工程.
  • 整形外科手术 整形外科手术

背景情况:

  • 软骨缺陷缺乏有效的治疗方法,推动了组织工程的研究.
  • 脱细胞化细胞外基质 (ECM) 是细胞生长的一个有前途的支架.
  • 基于洗剂的脱细胞化可能会损害ECM,阻碍再细胞化.

研究的目的:

  • 使用物理方法优化软骨脱细胞化.
  • 在脱细胞化过程中避免使用离子洗剂.
  • 为了创建一个生物相容和生物活性的软骨支架.

主要方法:

  • 牛气管软骨被用冷解周期和超声波去细胞化.
  • 组织被用0.25%的素处理.
  • 进行了组织学,细胞相容性和体内生物评估.

主要成果:

  • 组织学证实了有效的细胞去除和ECM保存.
  • 纤维细胞细胞培养表明成功的增殖和迁移.
  • 活体内研究显示免疫反应减少和白细胞透.

结论:

  • 物理脱细胞化有效地去除细胞,同时保持ECM.
  • 由此产生的支架适用于软骨重建.
  • 这种方法避免了有毒化学品,提高了脚手架的安全性.