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与MERS相关的冠状病毒HKU25类的ACE2利用,具有广泛的地理分布

Chen Liu¹, Young-Jun Park^2,3, Cheng-Bao Ma¹

¹State Key Laboratory of Virology and Biosafety, College of Life Sciences, TaiKang Center for Life and Medical Sciences, Wuhan University; Wuhan, Hubei, 430072, China.

Research square

|March 31, 2025

概括

滴基酶-4 (DPP4) 是一种已知的与MERS相关的冠状病毒 (MERSr-CoVs) 的受体. 这项研究揭示了一些蝙蝠MERSr-CoVs利用血管酶转化酶2 (ACE2) 作为其功能受体,扩大了我们对病毒进入机制的理解.

科学领域:

病毒学病毒学
分子生物学分子生物学
结构生物学结构生物学

背景情况:

双基酶-4 (DPP4) 是来自各种宿主的MERS相关冠状病毒 (MERSr-CoVs) 的已确定的受体.
不同蝙蝠MERSr-CoVs的受体使用仍然不完全理解.
ангиотензин转化酶2 (ACE2) 最近已经成为几个merbecovirus类的入口受体.

研究的目的:

为了研究遗传多样性的蝙蝠MERSr-CoVs的受体使用.
描述蝙蝠merbecoviruses与ACE2和DPP4.4的相互作用.
了解MERSr-CoV受体热带的进化动态.

主要方法:

使用病毒抗原和基于伪病毒的功能测试.
使用冷电子显微镜分析病毒与受体相互作用.
评估了ACE2的正经热带性和对中和抗体的敏感性.

主要成果:

一些来自HKU25类的蝙蝠merbecoviruses,以前认为使用DPP4,被发现可以利用ACE2.
化EM检测显示,HKU25类病毒与ACE2的结合模式是保留的,但是改造的,与HKU5.5不同.
EjCoV-3表现出广泛的ACE2正义位和低于最佳的人类ACE2利用率.

结论:

这项研究重新定义了对MERSr-CoVs受体使用演变的理解.
ACE2是各种冠状病毒的多功能功能受体,包括蝙蝠merbecoviruses.
尽管进入机制不同,但这些病毒对中和抗体和进入抑制剂表现出敏感性.

关键词:

在ACE2中,ACE2是ACE2.化电磁波是一种冷电磁波.DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 DPP4 这是EjCoV-3的原型.在HKU2525中使用.这就是MERSr-CoV.接收器接收器接收器

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