调节细胞内自和巨细胞酶,以增加邻近药物输送
Shuaipeng Feng1, Qingqing Xu1, Bin Liu1
1Department of Pharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, PR China.
ACS nano
|March 31, 2025
概括
细胞内自细胞通过增强的宏皮诺细胞分裂向邻近的瘤细胞输送药物. 这种策略,利用Ca2+化聚多巴胺纳米颗粒,改善药物透,抑制瘤生长和转移.
科学领域:
- 生物医学工程 生物医学工程
- 纳米技术 纳米技术
- 癌症治疗 癌症治疗
背景情况:
- 由于癌症相关纤维细胞 (CAF),纳米颗粒药物输送在深度瘤透方面面临挑战.
- 无法控制的药物转细胞和CAFs有限的吸收阻碍了有效的瘤向.
研究的目的:
- 开发一种新的策略,以使用细胞内自细胞和巨细胞解剖来增强深度瘤药物输送.
- 为了研究Ca2+化聚多巴胺纳米颗粒载有GLS1抑制剂 (CB-839) 和谷氨酸 (839/CG) 在触发这种传递机制中的作用.
主要方法:
- 用CB-839和谷氨酸 (839/CG) 装载的Ca2+化聚多巴胺纳米颗粒的制备.
- 使用839 / CG和Ca2 +释放诱导溶酶体损伤和自积累.
- 通过mTOR下调和谷氨胺饥饿刺激瘤巨细胞细胞形成.
- 结合疗法与光热效应,以减少CAF和促进重复的药物递送周期.
主要成果:
- 839/CG纳米颗粒有效触发了自细胞的积累,并增强了瘤细胞中的巨皮诺细胞形成.
- 释放的含有839 / CG的自细胞被邻近的瘤细胞积极摄入,促进瘤深入透.
- 光热疗法减少了CAFs,使药物递送的重复循环成为可能.
- 治疗诱导了免疫细胞死亡,增强了抗瘤免疫反应,减少了转移.
结论:
- 细胞内自细胞组合与增强的巨细胞细胞结合,代表了深度瘤药物输送的有希望的方法.
- 开发的839/CG纳米粒子有效地利用细胞机制来向药物释放和吸收.
- 这一策略有可能通过克服透障碍和增强免疫反应来提高癌症治疗效率.
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