Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Ras Gene02:38

The Ras Gene

6.1K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.1K
Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

3.8K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
3.8K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.3K
Rab Proteins01:14

Rab Proteins

3.8K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
3.8K
Experimental RNAi02:15

Experimental RNAi

6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Community-Based Exercise for Chronic Pain in People with Spinal Cord Injury: A Pragmatic Randomized Controlled Trial.

Archives of physical medicine and rehabilitation·2026
Same author

Targeting Multiple KRAS Mutations with High-Affinity Macrocyclic Inhibitors: From Discovery to Preclinical Validation.

Journal of medicinal chemistry·2026
Same author

The impact of augmented reality on radiation exposure during spine surgery: a systematic review.

Neurosurgical review·2026
Same author

Factors predicting MRI glioma segmentation accuracy in deep learning models: a systematic review and meta-analysis.

Journal of neuroradiology = Journal de neuroradiologie·2026
Same author

Correction: Dynamic conformational equilibria in the active states of KRAS and NRAS.

RSC chemical biology·2026
Same author

Assessing large language models in radiation risk communication: susceptibility, cultural-linguistic effects, and ethical reasoning.

International journal of radiation biology·2026
Same journal

Library Docking for Cannabinoid-2 Receptor Ligands.

Journal of medicinal chemistry·2026
Same journal

Charting New Territory: Systematic Evaluation of the Drug Potential of <i>N</i>-Trifluoromethyl Amides, Ureas & Carbamates.

Journal of medicinal chemistry·2026
Same journal

Red-Light-Triggered <i>In Vitro</i> and <i>In Vivo</i> Photocatalytic Cancer Therapy with Polypyridyl Os(II) Photocatalysts.

Journal of medicinal chemistry·2026
Same journal

Novel Selenium-Containing Small Molecule PD-L1 Inhibitors: Design, Synthesis, and Evaluation of the Antitumor Activity.

Journal of medicinal chemistry·2026
Same journal

HsClpP-Engaging Selective Mitochondrial Pan-PDK Degraders for Cancer Therapy.

Journal of medicinal chemistry·2026
Same journal

Rational Development of Activatable Prodrugs of the GSTP1 Inhibitor NBDHEX: Turn-On NIR Fluorogenic Drug Delivery with Selective Anticancer Activity.

Journal of medicinal chemistry·2026
查看所有相关文章

相关实验视频

Updated: May 16, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.8K

可逆小分子多变Ras抑制剂对Ras的活性和非活性形式显示可调节的亲和力.

Charles W Parry1, Francesca Pellicano1, Alexander W Schüttelkopf1

  • 1Cancer Research Horizons, CRUK Scotland Institute, Garscube Estate, Switchback Road, Glasgow G61 1BD, U.K.

Journal of medicinal chemistry
|March 31, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了针对Ras蛋白的新可逆抑制剂,为各种癌症提供了超越KRas G12C突变的潜在治疗方法. 这些抑制剂通过向活跃和非活跃的Ras状态来阻断癌症驱动途径具有前途.

更多相关视频

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.0K
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
06:44

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Published on: March 1, 2024

897

相关实验视频

Last Updated: May 16, 2025

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells
10:27

Spatio-Temporal Manipulation of Small GTPase Activity at Subcellular Level and on Timescale of Seconds in Living Cells

Published on: March 9, 2012

10.8K
Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
07:49

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Published on: July 17, 2019

6.0K
Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
06:44

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Published on: March 1, 2024

897

科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 药物发现 药物发现 药物发现

背景情况:

  • 激活Ras突变是癌症的关键驱动因素,经常与不良预后有关.
  • 目前的KRas G12C抑制剂针对特定的突变,留下了对其他Ras变体的重大未满足需求.
  • 针对非G12C Ras突变的有效性在很大程度上仍未被探索.

研究的目的:

  • 发现针对广泛Ras突变的新型可逆抑制剂.
  • 研究Ras蛋白质中结合扩大开关I-II口袋的抑制剂.
  • 评估这些抑制剂在阻断Ras驱动信号通路方面的潜力.

主要方法:

  • 设计和合成一系列新的可逆Ras抑制剂.
  • 生物化学测试以确定抑制剂的结合亲和性和状态 (活性/无活性).
  • 细胞测试以评估Ras-Raf相互作用的抑制和下游ERK酸化.

主要成果:

  • 确定了具有纳米分子亲和力的可逆抑制剂,用于扩大的Ras开关I-II口袋.
  • 开发出选择性结合非活性Ras,活性Ras或两种状态的化学型.
  • 证明活性状态结合剂抑制野生型Ras和多种瘤性KRas突变,阻断Ras-Raf相互作用并减少细胞中的ERK酸化.

结论:

  • 发现了一种新型的可逆Ras抑制剂类别,针对扩大的口袋.
  • 这些抑制剂对各种Ras突变具有广泛的活性,包括非G12C变种.
  • 这些发现为开发多变体Ras抑制剂来治疗各种癌症提供了一个有希望的新策略.