无菌菌对小麦粒的生长影响沙门氏菌和滋果毒素产生大肠杆菌的弹性
Yawei Lin1, Carolyn Peterson1, Bradley P Marks2
1Department of Food Science and Human Nutrition, Michigan State University, East Lansing, Michigan, USA.
Applied and environmental microbiology
|March 31, 2025
概括
伊诺库勒姆制剂对小麦粒的病原体生存有显著影响. 酸适应的滋毒素产生大肠杆菌 (STEC) 显示出更好的干燥耐受性,并且用抗微生物溶液炼有效降低了病原体负载.
科学领域:
- 食品微生物学 食品微生物学
- 食品安全 食品安全
- 细菌病原体的产生
背景情况:
- 麦粉爆发突显了需要了解小麦谷物的病原体生存动力学.
- 不一致的注射剂制备方法阻碍了对小麦炼处理的研究中的比较.
- 确定病原体污染的"最坏情况"对于评估食品安全干预措施至关重要.
研究的目的:
- 为了量化沙门氏菌和石毒素产生大肠杆菌 (STEC) 在小麦谷物上的干燥生存和化反应.
- 评估五种不同的注射剂制备方法对病原体生存的影响.
- 评估在小麦炼过程中化学干预的有效性,以减少病原体.
主要方法:
- 用五种不同的生长方法 (汁,草有氧,草无氧,草酸适应,草低等) 制备的沙门氏菌和STEC菌株对小麦粒进行注射.
- 在0.45水活性 (aw) 处存储注射小麦,最长可存储24周.
- 用水,,乳酸 (LA) 或二硫酸 (SBS) 化储存的小麦,然后进行病原体计数.
主要成果:
- 伊诺库勒姆的制备方法显著影响了病原体生存曲线和干燥耐受性.
- 适应酸的STEC表现出对干燥的增强耐受性;沙门氏菌没有表现出类似的适应性.
- 化疗法降低了病原体负载,而二硫酸 (SBS) 显示了最高的有效性 (1.96 log CFU/g的减少).
结论:
- 在小麦炼过程中,Inoculum的制备方法和储存时间会影响病原体的生存.
- 适应酸的注射剂通常会在炼过程中使病原体存活得更好.
- 在炼期间的抗菌干预提供了一种可行的策略,以减轻小麦中食物传播病原体的风险.
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