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适应性进化和逆向工程,以探索Streptomyces albulus的低pH容忍机制

Yuxi Liu¹, Tianyi Liu¹, Yulin Zhang¹

¹Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, China.

Applied and environmental microbiology

|March 31, 2025

概括

适应性实验室进化增强了Streptomyces albulus对低pH值的耐受性,提高了37.9%的e-poly-L-lysine (e-PL) 产量. 确定了参与细胞膜和细胞壁完整性的关键基因,提高了菌株活力和生物合成效率.

科学领域:

微生物学微生物学
生物技术是生物技术.
分子生物学分子生物学

背景情况:

藻是e-poly-L-lysine (e-PL) 的关键生产者,但其效率受到低pH要求的限制.
长时间暴露在酸性条件下会损害细胞完整性,减少S. albulus中的e-PL生物合成.

研究的目的:

通过适应性实验室进化 (ALE) 来提高S. albulus的低pH耐受性.
调查S. albulus.低pH耐受性的潜在机制.
在具有挑战性的pH条件下提高e-PL生产效率.

主要方法:

在逐渐降低pH值的情况下,S. albulus GS114菌株的适应性实验室进化 (ALE).
全基因组再测序和定量实时PCR用于识别关键基因.
逆向工程验证基因功能及其对低pH耐受性和e-PL产生的影响.

主要成果:

开发出一种突变菌株ALE3.6,具有显著改善的低pH耐受性 (pH 3.6).
与家长菌株相比,ALE3.6菌株的e-PL产量增加了37.9%.
确定了desA,gatD和mamU作为关键基因,有助于增强低pH耐受性和细胞膜/细胞壁完整性.

结论:

ALE是一种有效的策略,可以改善S. albulus低pH耐受性和e-PL生产.
已识别的基因 (desA,gatD,mamU) 在细胞对低pH压力的反应中起着至关重要的作用.
了解这些机制可以优化Streptomyces物种中有价值的生物化学品的工业生产.

关键词:

这是一种Streptomyces albulus.适应性实验室进化的演变.耐低pH的耐受性较低.反向工程是一种逆向工程.ε-多聚-L-氨酸是什么

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