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Updated: May 16, 2025

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多个细胞外聚合物物质路由由Accumulibacter和侧面社区在有氧颗粒形成期间和影响性修饰后转录
Laëtitia Cardona1, Jaspreet Singh Saini1, Pilar Natalia Rodilla Ramírez1
1Laboratory for Environmental Biotechnology, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Applied and environmental microbiology
|March 31, 2025
概括
有氧颗粒污泥的稳定性由各种微生物群落维持,这些微生物群落产生细胞外聚合物质. 即使减少了酸盐,这些社区,包括Propionivibrio,通过复杂的相互作用来确保颗粒的完整性.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 生物膜的形成过程.
背景情况:
- 有氧颗粒污泥 (AGS) 与传统活性污泥相比,提供更高的沉速度.
- AGS依赖于微生物群落,形成富含细胞外聚合物物质 (EPS) 的颗粒状生物膜.
- 了解微生物在EPS生产和颗粒稳定性中的作用对于优化废水处理至关重要.
研究的目的:
- 调查Candidatus Accumulibacter在AGS内部EPS生产中的作用.
- 分析微生物社区的新陈代谢和基因表达,使用 de novo metatranscriptomics.
- 为了确定减少影响酸盐对颗粒稳定性和微生物活性的影响.
主要方法:
- 在配序批量反应器中培养AGS,其中富含聚酸盐积累生物.
- 微生物基因表达和新陈代谢的全面分析.
- 控制降低影响酸盐度以评估其影响.
主要成果:
- 一个多样化的微生物群落,包括多个Candidatus Accumulibacter物种,主导了AGS.
- 新的元转录组学揭示了多样化的EPS生产,主要是通过Candidatus Accumulibacter,Azonexus,Candidatus Contendobacter和Propionivibrio.
- 减少的酸盐导致了Propionivibrio的主导地位,但保持了颗粒稳定性,这表明微生物之间的EPS循环利用.
结论:
- 颗粒形成是一种动态的微生物过程,涉及复杂的相互作用和多样化的EPS生产.
- 旁边的微生物群落积极促进EPS的合成和循环利用,增强矩阵稳定性.
- 稳定的有氧颗粒可以保持Propionivibrio主导的社区,即使在酸盐有限的条件下.
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