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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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在马来西亚出现B.1.617.2 (三角洲) 变种期间,SARS-CoV-2阳性样本中RT-PCR Ct值的下降.

N Che-Kamaruddin1, B T Teoh1, K K Tan1

  • 1Tropical Infectious Diseases Research and Education Centre (TIDREC), Higher Institution Centre of Excellence (HICoE), Universiti Malaya, 50603 Kuala Lumpur, Malaysia.

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概括

从RT-PCR测试中分析周期值 (Ct) 值可以揭示SARS-CoV-2传播动态. 2021年较低的CT值与三角洲变种 (B.1.617.2) 的出现以及马来西亚COVID-19病例的增加有关.

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科学领域:

  • 病毒学 病毒学
  • 流行病学 流行病学
  • 分子诊断学 分子诊断

背景情况:

  • 反转录-聚合酶链反应 (RT-PCR) 周期值 (Ct) 值与病毒载荷成反比例.
  • SARS-CoV-2 变种的出现,特别是 B.1.617.2 (三角形),显著影响了全球感染率.
  • 马来西亚在2021年经历了大量的COVID-19急剧增长,与德尔塔变种的流行情况相吻合.

研究的目的:

  • 分析Ct值与马来西亚SARS-CoV-2基因组变异相关的分布.
  • 调查Ct值,病毒变异和COVID-19流行趋势之间的相关性.
  • 评估Ct值分析对监测人口层面传播动态的有用性.

主要方法:

  • 从2020年和2021年对阳性COVID-19样本的CT值分布的表格.
  • 基因组测序用于识别SARS-CoV-2变种 (B.1.36,B.1.524,B.1.617.2). 基因组测序用于识别SARS-CoV-2变种 (B.1.36,B.1.524,B.1.617.2). 基因组测序用于识别SARS-CoV-2变种.
  • 对Ct值趋势的统计分析和与国家COVID-19病例数据的比较.

主要成果:

  • 从2020年到2021年,观察到中位数Ct值的显著下降 (p<0.01).
  • 2021年的样本主要包括B.1.617.2变种,与2020年变种相比,该变种的中位数Ct值较低.
  • 降低Ct值的趋势与马来西亚每周COVID-19病例的增加有关.

结论:

  • CT值分布分析可以有效地表明人口层面的SARS-CoV-2传播动态.
  • 该研究强调了B.1.617.2变种,较低的CT值和增加的COVID-19病例之间的联系.
  • 将CT值分析与基因组测序相结合,有助于早期检测和控制新出现的SARS-CoV-2菌株.