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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
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解读调节番茄基因型间诱导系统性抗性的变异性机制.

Jane Marian Luis1, Amit K Jaiswal2, Tesfaye D Mengiste3

  • 1University of Hawai'i at Manoa, Honolulu, Hawaii, United States; luisjm@hawaii.edu.

Phytopathology
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概括
此摘要是机器生成的。

番茄的基因型决定了它对Trichoderma harzianum,一种有益的真菌的反应. 这项研究揭示了关键的分子通路,比如布拉西诺斯特罗伊德和酸信号传递,这主要是对Botrytis cinerea耐药的番茄,改善了生物控制策略.

关键词:
生物控制 生物控制菌类病原体的真菌病原体一个分子分子的分子.植物的免疫反应.

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科学领域:

  • 植物病理学 植物病理学
  • 分子生物学分子生物学
  • 农业科学 农业科学

背景情况:

  • 番茄对叶子病原体的诱导系统性耐药性 (ISR) 受基因型的影响.
  • 特里科德玛哈尔齐安 (Trichoderma harzianum) 是一种用于生物控制的有益真菌.
  • Botrytis cinerea 是一个重要的西红叶病原体.

研究的目的:

  • 调查番茄中针对B. cinerea. 的ISR基因型特异性机制.
  • 为了比较响应性 (Solanum pimpinellifolium LA 1589) 和不响应性 (Solanum lycopersicum cv. cv. 威斯康星州55) 番茄的基因型.
  • 使用RNA-seq.识别涉及差异性ISR反应的分子途径.

主要方法:

  • 用T. harzianum对番茄基因型进行治疗,然后用B. cinerea.进行接种.
  • 现型分析包括疾病病变区域,植物高度和根生物量.
  • 使用RNA测序 (RNA-seq) 来比较基因型的时间基因表达概况.

主要成果:

  • 在S. pimpinellifolium LA 1589中,T. harzianum治疗增强了ISR,显示疾病减少,植物高度增加和根生物量增加.
  • 在S. lycopersicum cv.中使用. 威斯康星州55在T. harzianum治疗后没有表现出类似的ISR益处.
  • 不同的基因表达揭示了基因类型之间对自,过敏反应和代谢途径 (auxin,brassinosteroid,乙烯,flavonoid, jasmonic acid,phenylalanine,salicylic acid, sterol) 的独特丰富.
  • 勃拉西诺,烯和酸/乙烯信号的上调,以及酸信号的下调,是ISR响应基因型的特征.

结论:

  • 铜类,类和酸/乙烯信号通路对于针对B. cinerea. 的ISR响应性番茄基因型的原始化至关重要.
  • 对酸信号的下调有助于有效的ISR.
  • 这些研究结果为增强基于Trichoderma的生物控制和为改善生长和病原体耐药性选择基因型提供了洞察力.