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基于无细胞DNA的"衰老时钟"

Aleksandr V Sergeev^1,2, Olga V Kisil^3,4, Andrey A Eremin³

¹Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 119991, Russia. avsergeev@belozersky.msu.ru.

Biochemistry. Biokhimiia

|March 31, 2025

概括

衰老的生物标志物,如无细胞DNA (cfDNA),可以使用衰老钟来预测生物年龄. 分析cfDNA碎片学,拓学和表观遗传学为开发更准确的衰老时钟提供了一个有希望的方法.

科学领域:

生物遗传学生物遗传学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

衰老涉及系统的生理和分子变化,称为衰老的生物标志物.
衰老时钟是统计模型,将生物标志物与生物年龄联系起来,使健康评估和衰老率的确定成为可能.
血液中的无细胞DNA (cfDNA) 是潜在的衰老生物标志物的丰富来源.

研究的目的:

审查cfDNA碎片学,拓学和表观遗传学作为衰老生物标志物.
探索cfDNA分析在开发先进衰老时钟方面的潜力.
突出单分子测序和长期阅读技术在这个领域的实用性.

主要方法:

在衰老的背景下对cfDNA分析的当前文献的综述.
详细检查cfDNA碎片组学,拓学和表观遗传修饰.
讨论用于cfDNA分析的先进测序技术.

主要成果:

cfDNA表现出与生物年龄相关的明显的断片,拓和表观遗传特征.
这些cfDNA特征是衰老的有希望的生物标志物.
单分子测序与长读技术相结合,可以同时对多个cfDNA生物标志物进行分析.

结论:

cfDNA分析对开发精确的衰老时钟具有重大潜力.
未来的研究应该专注于利用先进的测序技术来进行全面的cfDNA生物标志物分析.
这种方法可以彻底改变对生物衰老和健康的定量评估.

关键词:

时钟的老化时间没有细胞的核酸.cfDNA DNA 的时间.显基因组是一个表观基因组.额外染色体的圆形DNA.一个碎片化的碎片.甲基化处理甲基化处理纳米孔测序的测序

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