温带菌体vB_SauS_S1对金黄色葡萄球菌ST398的适应性和致病性的影响
Hui Liu1,2, Craig Billington3, Xing Ji1
1Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P.R. China.
BMC microbiology
|April 1, 2025
概括
一种新型的菌体,vB_SauS_S1,有效地感染牲畜相关的金黄色葡萄球菌ST398. lysogenic 菌株表现出增强的生物膜形成和细胞入侵,抗生素敏感性或毒性没有显著变化.
科学领域:
- 微生物学 微生物学
- 病毒学 病毒学
- 细菌治疗疗法是一种细菌治疗.
背景情况:
- 与牲畜相关的金黄色葡萄球菌ST398是一种重要的动物传染病原体.
- 菌体 (菌体) 是感染细菌的病毒,具有治疗应用的潜力.
- 了解菌体与宿主之间的相互作用对于开发针对S. aureus ST398.8的新型控制策略至关重要.
研究的目的:
- 为了隔离和表征一种向金黄色葡萄球菌 (ST398.8) 的菌体.
- 调查菌体S1溶解酶对黄金色杆菌ST398.8特征的影响.
- 评估菌体S1作为研究S. aureus ST398进化和致病性的工具的潜力.
主要方法:
- 使用S. aureus ST398.8对菌体vB_SauS_S1的分离和表征.
- 基因组分析和遗传学分析来分类菌体S1.
- 在体外测试以评估动物模型 (Galleria mellonella和小鼠) 中的生物膜形成,细胞入侵,抗生素敏感性和毒性.
主要成果:
- 体vB_SauS_S1感染了73.33%的测试S. aureus ST398分离体,并被归类为温带体.
- Lysogenic S. aureus ST398菌株显示生物膜形成和MAC-T细胞入侵率增加.
- 素没有改变抗生素敏感性,也没有显著影响动物模型中的毒性;素获得了超级感染免疫力.
结论:
- 温的菌体S1可以感染和溶解S. aureus ST398,赋予增强的生物膜和入侵能力.
- 菌体S1 lysogeny提供了对其他菌体的超感染免疫力,这表明菌体耐药性的作用.
- 这项研究增强了对温带菌体和动物感染病原体S. aureus ST398.8之间的共同进化动态的理解.
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