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评估QuantiFERON结核病黄金加在高发病率设置中的结核病家庭接触者中
Sophie Nalukwago1, Bonnie Thiel2, Keith Chervenak2
1Joint Clinical Research Centre, Kampala, Uganda. snalukwago@jcrc.org.ug.
昆菲龙-结核病黄金加 (QFT-Plus) 和昆菲龙-结核病黄金管内 (QFT-GIT) 测试显示,在识别结核病感染方面存在显著一致. 在家庭接触者中,没有临床因素与QFT-Plus CD8+ T细胞反应有关.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 诊断 诊断 诊断 诊断
背景情况:
- 准确识别潜伏结核病 (TB) 感染对于有效的预防策略至关重要.
- 量子费隆-结核病黄金Plus (QFT-Plus) 试验使用两个抗原管来评估结核病家庭接触者的CD4+和CD8+T细胞反应.
研究的目的:
- 评估QFT-Plus和QuantiFERON-TB Gold In-Tube (QFT-GIT) 试验在结核病家庭接触者中的一致性.
- 调查QFT-Plus CD8+ T细胞反应与临床显著变量之间的潜在关联.
主要方法:
- 56个家庭结核病接触者使用QFT-Plus和QFT-GIT测试进行了测试.
- 在QFT-Plus TB2和TB1管之间分析了干扰素玛 (IFNγ) 水平,截止值为0.6 IU/mL.
- 从一项基因研究中评估了616个样本,以评估CD8+ T细胞反应和临床因素之间的关联.
主要成果:
- 科恩的卡帕值为0.71,表明QFT-Plus和QFT-GIT测试之间存在显著一致.
- 八名参与者在两项测试中显示出不一致的结果.
- QFT-Plus CD8+ T细胞反应与年龄,性别,BCG疫苗接种,艾滋病毒状况,结核病风险评分或基线血液抽取没有显著相关.
结论:
- 在评估结核病感染方面,QFT-Plus和QFT-GIT测试显示了实质性的一致性.
- 没有发现有临床意义的变量与通过QFT-Plus测量的CD8+T细胞反应有关.
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