ASFV pS183L蛋白通过阻断MDA5寡合化来负面调节RLR介导的抗病毒信号
Huan Chen1,2, Qun Yu1,2, Xiaoyu Gao1,2
1Laboratory of Emerging Infectious Diseases and One Health, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, China.
Veterinary research
|April 1, 2025
概括
一种新型蛋白质ps183L通过阻断MDA5寡合化来抑制病毒传感,这是视网膜酸诱导基因I (RIG-I) 类受体信号传递和随后的干扰素产生的一个关键步骤.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 网红酸诱导基因I (RIG-I) 类受体 (RLR) 对于检测病毒感染至关重要.
- RLRs的功能,特别是在DNA病毒感染中,需要进一步阐明.
研究的目的:
- 研究非特征蛋白pS183L在RLR信号通路中的作用.
- 确定pS183L如何影响干扰素-β (IFN-β) 激活,以应对病毒刺激.
主要方法:
- 在细胞模型中过度表达ps183L.
- 通过MDA5,RIG-I和cGAS-STING通路触发的IFN-β激活的评估.
- 对聚 (I:C) 诱导的IFN-β产生进行分析.
- 同免疫沉试验用于研究蛋白质与蛋白质相互作用.
主要成果:
- pS183L过度表达特别抑制了MDA5介导的IFN-β激活,而不会影响RIG-I或cGAS-STING通路.
- pS183L抑制了由高分子量聚 (I:C) 诱导的IFN-β产生,这是已知的MDA5激活剂.
- 发现pS183L与MDA5的CARDs和Helicase域相互作用,防止MDA5的寡合化.
- pS183L的相互作用阻止了MDA5和MAVS之间的下游相互作用.
结论:
- 蛋白质ps183L作为MDA5信号的负调节剂.
- pS183L通过直接的蛋白质-蛋白质相互作用来抑制MDA5的寡合化,从而破坏MDA5介导的先天免疫反应.
- 这项研究揭示了一种新的病毒免疫逃避机制,涉及ps183l.
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