通过双膜辅助的电荷标记,丰富和现场解 NanoESI-MS 进行增强的呼吸化分析
Beichen Zhu1, Yifan Wei1, Xiumei Zheng2
1Analytical & Testing Center, Sichuan University, Chengdu 610064, China.
Analytical chemistry
|April 1, 2025
概括
这项研究引入了一种新的标记探头,用于敏感检测出口气中的化物,这对于癌症诊断至关重要. 该方法使呼吸生物标志物的快速,超敏感分析成为可能,有助于早期发现癌症.
科学领域:
- 分析化学 分析化学
- 生物标志物发现发现
- 质谱测量质量谱测量
背景情况:
- 呼出的气息中的化物是癌症诊断的关键挥发性有机化合物 (VOC) 生物标志物.
- 目前使用喷雾式电离质谱的检测方法在灵敏度和速度方面面临挑战.
- 在复杂的呼吸矩阵中灵敏和快速检测化物生物标志物仍然是一个重大障碍.
研究的目的:
- 开发一种新,灵敏和快速的方法来检测出口气中的化物生物标志物,用于癌症诊断.
- 引入充电的同位素签名探针,以有效捕获和识别气相化物.
- 验证该方法在量化出口气中的化物中的有效性,并将癌症患者与健康个体区分开来.
主要方法:
- 用一种有反应性氨基部分的标记探头固定在多孔的尼龙-6膜上,以捕获和丰富化物.
- 以溶剂快速提取阿尔德衍生物,然后使用氧化纤维素膜进行净化.
- 超敏感的纳米电子喷雾电离质谱 (nanoESI-MS) 分析使用复合体内部标准进行量化.
主要成果:
- 开发的方法实现了超灵敏的化物检测,其极限低于每万亿分之0.1 (ppt).
- 在呼出的呼吸样本中成功识别和量化了12种不同的化物.
- 与健康对照人群相比,在食道癌症患者中观察到特定化物的显著升高.
结论:
- 标记探头和纳米ESI-MS方法提供了一个快速,准确和超灵敏的方法来检测呼气中的化物生物标志物.
- 这种技术显示出作为早期癌症诊断,特别是食道癌症的非侵入性工具的显著前景.
- 该研究强调了针对临床应用的先进质谱仪的有针对性呼吸分析的潜力.
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