在mgrB中的插入序列和两个组件系统基因中的突变赋予了对抗卡巴胺耐药的Enterobacter cloacae复杂菌株的高聚素耐药性
Jiming Wu1, Jisheng Zhang1, Jianmin Wang1
1Department of Microbiology, Yongchuan Hospital of Chongqing Medical University, Chongqing, China.
Frontiers in microbiology
|April 1, 2025
概括
鉴定出了耐卡巴胺的Cloacae Enterobacter复合体 (CRECC) 菌株,其中一些携带mcr-9基因. 在CRECC中的多素B耐药性是复杂的,涉及mcr-9和phoP/Q和mgrB的突变.
科学领域:
- 微生物学 微生物学
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
背景情况:
- 在物种层面上很难识别Enterobacter cloacae复合体 (ECC),这限制了对抗卡巴胺ECC (CRECC) 分布的理解.
- 等离子体介导的mcr基因有助于多素耐药性,mcr-9在CRECC中成为一个关注点.
研究的目的:
- 为了确定CRECC和mcr-9基因的流行率.
- 调查CRECC中高水平多素B耐药性的机制.
主要方法:
- 收集并分析了212个ECC菌株.
- 确定了CRECC菌株,并对mcr-9基因进行了选.
- 分析了phoP/Q和mgrB基因中的突变,并进行了蛋白质结构预测.
主要成果:
- 确定了38种CRECC菌株 (17.9%),其中Enterobacter hormaechei占主导地位.
- 在10个CRECC菌株 (36.3%) 中发现mcr-9;它的存在没有显著影响聚米辛B耐药性.
- 由于phoP/Q和mgrB的突变,在17个CRECC菌株中观察到多素B的抗性增加,包括mgrB中的新插入序列IS5D和IS1X2.
结论:
- 在CRECC中,多素B耐药性是多因素的,涉及mcr-9和染色体机制.
- mgrB 无活化和 phoP/Q 突变显著导致多素 B 耐药性.
- 在mgrB中新的插入序列突出了抗生素耐药性演变的动态性质.
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