相关实验视频

Updated: May 16, 2025

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

用MexT调节的因子调节Pseudomonas aeruginosa的定数感应级联

Andrew Frando¹, Robert S Parsek², Jamal Omar²

¹Department of Medicine, University of Washington, Seattle, WA 98195 USA.

bioRxiv : the preprint server for biology

|April 1, 2025

概括

Pseudomonas aeruginosa 的定量感应 (QS) 调节由 MexT 调节,通过 MexEF-OprN 和 PQS 合成影响 QS 层次. 这揭示了新的QS调节器影响细菌行为.

科学领域:

微生物学微生物学
细菌的沟通方式
基因调节基因调节

背景情况:

Pseudomonas aeruginosa (Pa) 使用定数感应 (QS) 进行细胞间通信和基因表达控制.
帕具有三个QS电路 (LasR,RhlR,PqsR),在PAO1菌株中具有层次组织,其中RhlR活动取决于LasR.
非QS转录因子MexT影响了这个QS层次结构,它的缺失使得RhlR活动独立于LasR.

研究的目的:

为了阐明MexT如何调节Pseudomonas aeruginosa QS架构.
为了确定影响QS等级的MexT调控基因.
在临床隔离中研究替代QS架构.

主要方法:

对PAO1和PAO1ΔmexT菌株进行比较转录组分析.
标识和表征的MexT规则.
在QS等级上对MexEF-OprN和PQS合成基因产物的实验测试.
在临床隔离中分析QS架构.

主要成果:

MexT 调节了 mexEF-oprN 和 pqsABCDE 操作符.
一种mexEF淘汰突变体表现出较早和更高的RhlR活性,类似于一个mexT删除突变体.
外源添加PQS也增强了RhlR活动,表明MexEF-OprN和PQS在QS调制中的作用.
临床分离显示了QS架构的替代性,其中MexT没有影响LasR-RhlR关系.

结论:

墨西哥T通过调节MexEF-OprN排泄和PQS合成来影响Pseudomonas aeruginosa QS等级.
这些发现揭示了影响帕州QS的新型监管因素.
该研究突出了多样化的QS架构和监管机制,对环境和临床环境都有影响.

关键词:

拉斯拉斯RR 的时间在PqsRR中使用.这是一个RhlRR.流量流量流量流量议员数量检测是指对议员数量进行检测.

更多相关视频

Author Spotlight: Studying Biomechanics of Circulating Cells by Modulating Their Electrodeformation Behavior

Author Spotlight: Studying Biomechanics of Circulating Cells by Modulating Their Electrodeformation Behavior

Published on: October 13, 2023

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Published on: May 29, 2014

相关概念视频

Regulation of Metabolism

Regulation of Metabolism

Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

Regulation of Expression at Multiple Steps

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...

Allosteric Regulation

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...

Regulation of Hormone Secretion

Regulation of Hormone Secretion

Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...