双链DNA病毒可以作为输送器,横向转移产生内子的转子子
Landen Gozashti1,2, Russell Corbett-Detig3,4
1Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA 02138.
bioRxiv : the preprint server for biology
|April 1, 2025
概括
双链DNA病毒在真核生物中促进了内部水平基因转移 (HGT). 这些病毒携带引子生成的转子子,可能会在物种中传播它们,并引入新的引子.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 进化生物学 进化生物学
背景情况:
- 在真核生物中,内增益受到称为内子的特殊可转移元素的显著影响.
- 水平基因转移 (HGT) 是塑造这些内基因分布的关键机制.
- 已知将病毒集成到宿主基因组中并调解HGT的病毒是内核转移的潜在载体.
研究的目的:
- 为了研究病毒作为载体的作用,在dinoflagellates中对内HGT进行研究.
- 为了识别和表征在dinoflagellate基因组中集成的病毒元素内的内子序列.
主要方法:
- 在多种类型的恐龙体基因组中整合病毒元素的注释.
- 对病毒元素进行系统的查询,以检测内核序列的存在.
- 与宿主基因组内基因相比,病毒内基因的年龄确定.
主要成果:
- 大约25%的注释病毒元素含有内子.
- 大多数这些病毒元素被识别为分离型或巨型双链DNA (dsDNA) 病毒.
- 在具有转移和病毒感染机制的全长病毒元素中发现了introners.
- 大多数病毒内置体比宿主内置体年轻或年龄相似,这表明获得后集成.
- 一个病毒元素的子集显示了显着较老的内部子,表明潜在的病毒对宿主HGT.
结论:
- 双链DNA病毒,特别是马维里克-波林顿类和巨型dsDNA病毒,作为内基HGT的重要载体.
- 病毒集成和随后的转移可以将内基因引入新宿主基因组.
- 病毒有助于在个体和物种之间传播引子生成的转子子,从而在真核细胞系中增加引子.
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