通过Campylobacter jejuni有效的胃肠道殖民需要ChuABCD血运输系统的组件
bioRxiv : the preprint server for biology
|April 1, 2025
概括
坎皮洛巴克特 (Campylobacter jejuni) 使用血红素运输系统来获取铁. 这种系统对于有效的肠道殖民和小鼠疾病发展至关重要,尽管不是完全必要的.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 细菌病原体的产生
背景情况:
- 坎皮洛巴克特 (Campylobacter jejuni) 具有一种利用系统,用于在铁含量有限的情况下生长.
- 这个系统的转录在人类感染期间被诱导.
- 这种对宿主殖民和疾病的海姆运输系统的必要性以前是未知的.
研究的目的:
- 研究运输系统在C. jejuni殖民和病原发生中的作用.
- 为了确定血红素运输系统的哪些组件对于血红素依赖的生长和铁的吸收至关重要.
- 在小鼠模型中评估血红素运输突变对肠道殖民,免疫激活和病理学的影响.
主要方法:
- 构建个别的非极性缺失突变体,用于体运输系统的每个组成部分.
- 为内部膜输送器ChuBCD创建一个全删除突变体.
- 在体外对血红素依赖生长和铁吸收的评估.
- 感染IL-10-/-小鼠以评估肠道殖民,便分泌,免疫细胞招募和肠道病理.
主要成果:
- 只有血红受体ChuA对依赖血红受体的生长和铁的获取至关重要.
- 血红素运输突变体在受感染小鼠的盲体和结肠中显著减少了便分泌和殖民.
- 中性细胞和巨细胞的招募,以及肠道病理,尽管减少了细菌负载,但仍然处于中等水平.
结论:
- 通过C. jejuni的血利用显著促进了高效的殖民和完全的致病性.
- 血红素运输系统很重要,但对于C. jejuni的殖民和疾病来说并不绝对必要.
- 向血利用可能是减少C. jejuni感染的策略.
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