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Updated: May 16, 2025

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

微流体纳米等离子体成像平台用于净化和无标签的单个小细胞外囊细胞计数.

Omid Mohsen Daraei, Avinash Kumar Singh, Saswat Mohapatra

bioRxiv : the preprint server for biology

|April 1, 2025

概括

这项研究引入了一种新的微流体芯片,用于无标签,单个小细胞外囊泡 (sEV) 在血中计数. 这种无净化方法为疾病诊断提供了敏感和有效的循环sEV检测.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
临床诊断临床诊断临床诊断

背景情况:

循环的小细胞外囊泡 (sEVs) 是有希望的疾病诊断的非侵入性生物标志物.
在复杂血中对sEV的定量检测具有挑战性,通常需要大量的样本准备.
目前用于sEV分析的方法可能耗时且容易出现手动错误.

研究的目的:

开发一种无净化方法,用于在血中进行敏感和高效的单个sEV计数.
为了证明微流体芯片与Plasmonic纳米孔径无标签成像 (PANORAMA) 集成的微流体芯片对于sEV检测的实用性.
建立一个快速和自动化的平台来分析流通中的SEV.

主要方法:

一个微流体芯片功能化CD63,CD9和CD81抗体在无形基板上排列的金纳米盘上 (AGNIS) 已开发用于选择性SEV捕获.
该芯片与PANORAMA集成,用于无标签,单次sEV计数.
一个自动化平台允许精确控制流量参数,并在60分钟内对20μL血样本进行分析.

主要成果:

开发的平台实现了无标签,单个sEV直接从血中计数,无需净化.
该系统在检测循环中的sEV方面表现出高灵敏度和效率.

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Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis

Published on: January 7, 2019

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Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

Published on: May 24, 2019

自动化微流体方法最大限度地减少了手动错误,并标准化了分析过程.

结论:

这种与 PANORAMA 集成的无净化微流体芯片提供了一种灵敏而高效的工具,用于检测循环中的 sEV.
该平台具有通过sEV生物标志物分析推进非侵入性疾病诊断的巨大潜力.
对小型血体积的自动化,快速分析为临床应用提供了切实可行的解决方案.