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Updated: May 16, 2025

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通过基因编码的模块化光探针进行循环的功能和结构分析

Marta Vittani1, Ashley Bomin Lee1, Xiaowen Wang1

  • 1Center for Translational Neuromedicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

bioRxiv : the preprint server for biology
|April 1, 2025
PubMed
概括

我们开发了一种用于实时流体跟踪的新鼠标模型. 该工具使用光标签来研究血液和间歇液体动态以及血液-大脑屏障的完整性 in vivo.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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