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相关概念视频

DNA Replication02:40

DNA Replication

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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
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The DNA Replication Fork01:02

The DNA Replication Fork

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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The Replisome03:01

The Replisome

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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Replication in Prokaryotes01:32

Replication in Prokaryotes

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DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
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Replication in Eukaryotes01:29

Replication in Eukaryotes

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In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
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相关实验视频

Updated: Jun 16, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

注册报告:复制和扩展的复制和扩展.

Karli M Nave, Erin E Hannon, Joel S Snyder

    bioRxiv : the preprint server for biology
    |April 1, 2025
    PubMed
    概括
    此摘要是机器生成的。

    复制研究发现,想象音乐节拍的神经效应比之前报道的要小. 这表明需要大样本大小才能使用频率标记可靠地检测节拍感知.

    关键词:
    听觉神经科学 听觉神经科学节拍和计时器的感知.频率标记的标记是一个频率标记.多个实验室的多个实验室音乐认知 音乐认知预注册 预注册 预注册 预注册复制复制复制复制复制复制复制

    更多相关视频

    Massively Parallel Reporter Assays in Cultured Mammalian Cells
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    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
    06:40

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

    Published on: March 22, 2018

    相关实验视频

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    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
    07:27

    Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

    Published on: April 29, 2010

    Massively Parallel Reporter Assays in Cultured Mammalian Cells
    11:03

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    Published on: August 17, 2014

    G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
    06:40

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    Published on: March 22, 2018

    科学领域:

    • 认知神经科学 认知神经科学
    • 听觉感知是一种听觉感知.
    • 音乐的神经科学 音乐的神经科学

    背景情况:

    • 区分刺激驱动和神经活动的有意识处理是一个长期存在的挑战.
    • 之前关于神经处理音乐节拍感知的研究可能会被刺激驱动的活动所混.
    • 频率标记提供了一种方法,通过测量特定频率的脑活动来分离节拍感知的神经相关物.

    研究的目的:

    • 进行Nozaradan等人13个独立的概念复制. (2011) 验证了对想象中的音乐节拍的神经处理的发现.
    • 调查观察到的神经效应是否可重复,并受到音乐或舞蹈训练的影响.
    • 评估频率标记的可靠性,以研究意识节拍感知的神经基础.

    主要方法:

    • 采用注册报告协议,对频率标记研究进行了13次独立复制.
    • 参与者在听同时刺激的同时执行听觉图像任务.
    • 整合了行为任务来测量有意识的感知和分析神经活动,使用元分析和后勤回归.

    主要成果:

    • 与原始研究相比,复制研究为与想象的节拍相关的神经活动产生了显著较小的效果大小.
    • 音乐和舞蹈训练并没有缓解观察到的神经效应.
    • 只有刺激频率的神经活动,而不是与图像相关的频率,预测了任务性能,挑战了原始研究的解释.

    结论:

    • 这些发现表明,频率标记可能需要非常大的样本大小来可靠地检测节拍感知的神经相关物.
    • 结果质疑先前发现的稳定性和频率标记在研究意识节拍感知方面的实用性.
    • 这些差异凸显了认知神经科学中严格复制的需要,以及研究感知意识的精细方法.