相关实验视频

Updated: Jun 26, 2026

DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

104.6K

广泛使用的二硫酸盐测序方法和DNA甲基化读取映射软件的可变性能

Emily V Kerns, Jesse N Weber

    bioRxiv : the preprint server for biology
    |April 1, 2025
    PubMed
    概括

    在自然种群中评估DNA甲基化 (DNAm) 需要仔细选择生物信息学工具. 对于DNAm分析,BWA meth提供了比Bismark或BWA mem更高的映射效率.

    更多相关视频

    Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain13:11

    Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

    Published on: July 12, 2012

    18.7K
    Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution13:47

    Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

    Published on: February 24, 2015

    25.5K

    相关概念视频

    DNA Microarrays02:34

    DNA Microarrays

    Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
    Sanger Sequencing01:57

    Sanger Sequencing

    DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
    RNA-seq03:21

    RNA-seq

    RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
    Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
    Maxam-Gilbert Sequencing01:05

    Maxam-Gilbert Sequencing

    In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
    Challenges of the Maxam-Gilbert Method
    The...
    Modern Molecular Taxonomy01:29

    Modern Molecular Taxonomy

    Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...