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前体作为人类微生物群中抗菌素耐药性基因的来源

Laura K Inglis¹, Susanna R Grigson¹, Michael J Roach^1,2

¹Flinders Accelerator for Microbiome Exploration, College of Science and Engineering, Flinders University, Bedford Park, SA, 5042, Australia.

bioRxiv : the preprint server for biology

|April 1, 2025

概括

菌体,细菌中的病毒,可以传播抗微生物耐药性 (AMR) 基因. 我们对人类细菌基因组的分析揭示了prophages内的AMR基因,突出了它们在抗药性转移中的作用.

科学领域:

微生物学微生物学
基因组学就是基因组学.
病毒学病毒学

背景情况:

前体是细菌基因组内集成的病毒元素,在微生物生态系统中普遍存在.
这些元素在水平基因转移中发挥着重要作用,可能会调解抗菌素耐药性 (AMR) 基因的传播.
对于人类微生物组研究和临床应用来说,了解菌体在抗药性抵抗中的参与至关重要.

研究的目的:

调查人类微生物群中携带AMR基因的prophages的流行率和多样性.
在与人类相关的细菌中识别特定的AMR基因和prophage-host关联.
评估AMR编码的prophages的地理分布和体位分布.

主要方法:

分析了近15,000个来自不同人体部位和地理位置的细菌基因组,来源于GenBank.
在细菌基因组内生物信息识别和特征的prophage序列.
检测和分类的抗微生物耐药性基因庇护由prophages及其宿主.

主要成果:

在分析的细菌基因组中,在6.6%的细菌基因组中发现了抗微生物耐药性基因,在有症状的个体中频率更高.
在菌体内发现了广泛的AMR基因,包括以前与等离子体相关的基因.
发现了新型的prophage-AMR基因关联,例如Acinetobacter baumannii中的"blaOXA-23"和Gardnerella物种中的"mefA-msrD",这表明prophage介导的转移.
携带AMR基因的原体在体位上表现出不同的频率,亚洲观察到的最高多样性.

结论:

前体是人类微生物群中抗菌素耐药性基因传播的重要载体.
基因中AMR基因的存在强调了它们对治疗策略的潜在临床影响.
进一步研究prophage-host-AMR相互作用是有必要的,以应对抗微生物药物耐药性日益增长的威胁.

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