在现场,单细胞生物打印有图案的前血管组织到生物打印的高密度干细胞装载的微凝生物墨水中,用于血管化骨组织再生
bioRxiv : the preprint server for biology
|April 1, 2025
概括
新的干细胞封装酸微凝 (SSAM) 生物墨水提供了改进的3D生物打印. 这些先进的生物墨水使血管化骨组织结构的高分辨率打印能够在现场植入.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 微凝越来越多地被用于3D生物打印,因为它们具有独特的特性,如剪切稀释和自我愈合.
- 目前生产细胞封装微凝的现有方法往往复杂,耗时,并且难以保持一致的质量和可扩展性.
研究的目的:
- 开发一种新的,可扩展的方法来制造具有理想印刷特性的干细胞封装微凝 (SSAM) 生物墨水.
- 展示SSAM生物墨的实用性,用于创建复杂的3D生物打印结构,包括血管前网络.
- 评估SSAM生物墨水在骨缺陷中血管化骨质组织的现场生物打印方面的潜力.
主要方法:
- 使用螺旋混合装置制造干细胞封装,可光交联,剪切稀释和自我愈合的酸微凝 (SSAM) 生物墨水.
- SSAM生物墨的3D生物打印使其成为具有高分辨率和形状保真性的复杂结构.
- 使用 SSAM 构造作为仅用于细胞的生物墨水打印的支浴,以创建血管前网络.
- 二次光交叉连接用于结构稳定和随后的体外分化到血管化骨质组织.
- 在先血管化组织的现场生物打印构建成小鼠骨缺陷.
主要成果:
- 通过简单的混合工艺,成功制造具有可调节性质的SSAM生物墨水.
- 复杂结构的高分辨率3D生物打印,由于剪切稀释和自我愈合特性,具有出色的形状保真性.
- 展示SSAM作为有效的支持浴室,用于在印刷结构中创建单细胞前血管网络.
- 在长期培养和二次光交联后实现了功能性血管化骨质组织形成.
- 成功地将先导体化构造物用于小鼠形缺陷的现场生物打印,显示出机械稳定性和缺陷中的保留.
结论:
- 开发的SSAM生物墨水为先进的3D生物打印应用提供了一个可扩展和高效的平台.
- 结合SSAM生物墨水和单细胞生物墨水,可以创建复杂的,血管化的组织结构.
- 该技术可在现场进行功能性,血管化的骨质组织的生物打印,用于骨再生,证明了机械稳定性和缺陷部位保留.
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