脑皮下脑区的三光子群体成像
Hadas Frostig1, Amy Monasterio2, Hongjie Xia3
1Department of Psychological and Brain Sciences, Boston University, Boston, Massachusetts, USA.
bioRxiv : the preprint server for biology
|April 1, 2025
概括
研究人员开发了一种大型成像视野三光子 (LIFT) 显微镜,以克服深部脑部成像方面的局限性. 这项新技术可以对老鼠和老鼠大脑中的1500多个细胞进行高分辨率成像.
科学领域:
- 神经科学是一个神经科学.
- 生物医学工程 生物医学工程
- 光学成像技术的成像
背景情况:
- 在深层大脑电路中记录神经活动对于理解大脑功能至关重要.
- 三光子 (3P) 成像为皮层下成像提供了潜力,但由于组织加热和低重复率,视野小 (FOV) 受到限制.
- 目前的3P成像FOVs被限制在≤0.3mm2.
研究的目的:
- 介绍一款新型显微镜,大成像视野三光子 (LIFT) 显微镜,能够显著扩大FOV用于深部大脑成像.
- 为了使大脑深部区域的人口成像能够具有高的信号噪声比.
- 在动物模型中使用非侵入性成像来证明LIFT显微镜的能力.
主要方法:
- 开发了LIFT显微镜,具有>3mm2的FOV,使用高数值孔径 (NA) 优化采样和定制扫描模块.
- 集成基于深度学习的除算法,以提高图像质量.
- 用LIFT显微镜在小鼠和老鼠大脑中进行非侵入性成像的应用.
主要成果:
- 在老鼠大脑区域 (CA1,白质,深层皮层) 展示了超过1500个细胞的非侵入性成像.
- 在大鼠皮层的1.2毫米深度实现了高信号对噪声人口成像.
- LIFT显微镜使用现成的组件,在成像规模和速度方面提供灵活性.
结论:
- LIFT显微镜显著扩大了在深层大脑结构中的三光子成像视野.
- 这项技术可以在大脑深层区域进行大规模的神经群体记录,从而推动神经科学研究.
- LIFT显微镜为深度大脑成像研究提供了一个灵活和可访问的平台.
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